製品の概要

  • 製品名

    Cell Lysis Buffer
  • アプリケーション

    適用あり: WB, IPmore details
  • 特記事項

    ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.

    Recommended for preparing samples for use in western blotting and immunoprecipitation. For use with adherent or suspension cells.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Store at -20°C. Stable for 12 months at -20°C
  • バッファー

    pH: 7.50
    Constituents: 0.29% Sodium EDTA, 1.12% Sodium pyrophosphate decahydrate, 8.8% Sodium chloride, 3.15% Tris HCl, 10% Triton-X-100, 0.38% EGTA, 0.001% Leupeptin, 0.19% Sodium orthovanadate, 0.216% Beta glycerophosphate
  • Concentration information loading...
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab152163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

プロトコール

参考文献

This product has been referenced in:

  • Raikwar SP  et al. Targeted Gene Editing of Glia Maturation Factor in Microglia: a Novel Alzheimer's Disease Therapeutic Target. Mol Neurobiol N/A:N/A (2018). Read more (PubMed: 29704201) »
  • Isaac C  et al. Mycolactone displays anti-inflammatory effects on the nervous system. PLoS Negl Trop Dis 11:e0006058 (2017). Read more (PubMed: 29149212) »
See all 7 Publications for this product

レビューと Q&A

Answer

For ab126615, I have attached the WB image with HepG2 cell lysate.

As for the cell lysis buffer used for the WB with ab108315, it is product ab152163 (https://www.abcam.com/index.html?datasheet=152163) that was used. The buffer composition is given on the datasheet:
pH: 7.50
Constituents: 0.216% Beta glycerophosphate, 0.19% Sodium orthovanadate, 0.001% Leupeptin, 0.38% EGTA, 10% Triton-X-100, 3.15% Tris HCl, 8.8% Sodium chloride, 0.29% Sodium EDTA, 1.12% Sodium pyrophosphate decahydrate

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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