Anti-CEACAM1 抗体 [EPR4048] - BSA and Azide free (ab179460)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4048] to CEACAM1 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CEACAM1 antibody [EPR4048] - BSA and Azide free
CEACAM1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4048] to CEACAM1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human CEACAM1 aa 50-150 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HepG2 cell lysate, A549 cell lysate, Human fetal liver tissue lysate, Human liver tissue and His-tagged human CEAM-1 fragment. IHC-P: Human kidney tissue, HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag and HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag.
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特記事項
ab179460 is the carrier-free version of ab108390.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4048 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179460の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. |
ターゲット情報
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配列類似性
Belongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact. - Information by UniProt
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参照データベース
- Entrez Gene: 634 Human
- Omim: 109770 Human
- SwissProt: P13688 Human
- Unigene: 512682 Human
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製品の状態
There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S. -
別名
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
see all
画像
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All lanes : Anti-CEACAM1 antibody [EPR4048] (ab108390) at 1/1000 dilution
Lane 1 : His-tagged human CEAM-1 fragment
Lane 2 : His-tagged human CEAM-3 fragment
Lane 3 : His-tagged human CEAM-5 fragment
Lane 4 : His-tagged human CEAM-6 fragment
Lane 5 : His-tagged human CEAM-8 fragment
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Additional bands at: 180 kDa (possible cross reactivity), 25 kDa (possible cross reactivity)
Exposure time: 37 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
This antibody cross-reacts with CEAM-3 and CEAM-5.
This data was developed using the same antibody clone in a different buffer formulation (ab108390).
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Immunohistochemical analysis of paraffin-embedded sections (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag. (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag. (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag. (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag. (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag. (F) HEK-293T cells transfected with empty vector containing a his tag.
This was labelling CEACAM1 with ab108390 at 1/2000 dilution. The section was incubated with ab108390 for 30 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counter stained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Positive staining on (A) HEK-293T cells transfected with a CEACAM1 expression vector containing a his tag, (B) HEK-293T cells transfected with a CEACAM3 expression vector containing a his tag and (C) HEK-293T cells transfected with a CEACAM5 expression vector containing a his tag. No staining on (D) HEK-293T cells transfected with a CEACAM6 expression vector containing a his tag, (E) HEK-293T cells transfected with a CEACAM8 expression vector containing a his tag and (F) HEK-293T cells transfected with empty vector containing a his tag. The results indicated cross reaction with CEACAM1, CEACAM3 and CEACAM5.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation (ab108390).
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All lanes : Anti-CEACAM1 antibody [EPR4048] (ab108390) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab108390).
Lanes 1 - 2: Merged signal (red and green). Green - ab108390 observed at 125 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108390 was shown to react with KDM5C / Jarid1C / SMCX in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108390 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab108390, at 1/100 dilution, staining CEACAM1 in Human kidney tissue by immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108390).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108390, at 1/100 dilution, staining CEACAM1 in Human liver tissue by immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108390).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab179460 は論文での使用が確認できていません。