アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    cdyl-antibody-ab5188.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
Share by email

Anti-CDYL 抗体 (ab5188)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (12)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-CDYL antibody (ab5188)
  • Western blot - Anti-CDYL antibody (ab5188)

Key features and details

  • Rabbit polyclonal to CDYL
  • Suitable for: IHC-P, ChIP, WB, ChIP-sequencing
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
Product image
Anti-CENPE antibody [1H12] (ab5093)

関連製品

製品の概要

  • 製品名

    Anti-CDYL antibody
    CDYL 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to CDYL
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, ChIP, WB, ChIP-sequencingmore details
  • 種交差性

    交差種: Mouse, Rat, Human, African green monkey
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5188の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
ChIP (1)
Use at an assay dependent concentration. PubMed: 22009739
WB (1)
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).
ChIP-sequencing
Use at an assay dependent concentration. PubMed: 22009739
特記事項
IHC-P
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration. PubMed: 22009739
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).
ChIP-sequencing
Use at an assay dependent concentration. PubMed: 22009739

ターゲット情報

  • 機能

    Acts as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4.
  • 組織特異性

    Ubiquitous. Expressed at moderate levels in all tissues examined.
  • 配列類似性

    Contains 1 chromo domain.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession Q9Y232 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 9425 Human
    • Entrez Gene: 12593 Mouse
    • Entrez Gene: 361237 Rat
    • Omim: 603778 Human
    • SwissProt: Q9Y232 Human
    • SwissProt: Q9WTK2 Mouse
    • SwissProt: Q6AYK9 Rat
    • Unigene: 269092 Human
    • Unigene: 29002 Mouse
    • Unigene: 146844 Rat
    see all
  • 別名

    • CDY-like antibody
    • CDY-like autosomal antibody
    • Cdyl antibody
    • CDYL: chromodomain protein Y-like antibody
    • CDYL1 antibody
    • CDYL1_HUMAN antibody
    • chromodomain protein Y chromosome-like 6 antibody
    • Chromodomain protein Y like antibody
    • Chromodomain Y-like protein antibody
    • DKFZP586C1622 antibody
    • MGC131936 antibody
    • OTTHUMP00000015984 antibody
    • OTTHUMP00000211185 antibody
    • OTTHUMP00000211186 antibody
    • OTTHUMP00000211189 antibody
    • testis-specific chromodomain Y-like protein antibody
    • Weakly similar to enoyl-CoA hydratase [H.sapiens] antibody
    see all

画像

  • Western blot - Anti-CDYL antibody (ab5188)
    Western blot - Anti-CDYL antibody (ab5188)
    All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
    Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?
    Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • Western blot - Anti-CDYL antibody (ab5188)
    Western blot - Anti-CDYL antibody (ab5188)Cecile Lestrat, Saadi Khochbin lab, Grenoble, France
    All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid

    Predicted band size: 66 kDa
    Observed band size: 83 kDa why is the actual band size different from the predicted?



    Rabbit polyclonal to CDYL (ab5188) at 1/1000.

    Left lane (-) COS7 (monkey) cells.
    Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.

プロトコール

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (12)

ab5188 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5188 は 12 報の論文で使用されています。

  • Tian H  et al. EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8. Mol Biol Cell 32:1-14 (2021). PubMed: 33175657
  • Pham V  et al. Proteomic Analyses Identify a Novel Role for EZH2 in the Initiation of Cancer Cell Drug Tolerance. J Proteome Res 19:1533-1547 (2020). PubMed: 32159963
  • Qiu Z  et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
  • Wang J  et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
  • Qi C  et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci 34:4494-508 (2014). Rat . PubMed: 24671995
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

ChIP abreview for Anti-CDYL antibody - ChIP Grade

Average
Abreviews
Abreviews
abreview image
Application
ChIP
Detection step
Real-time PCR
Sample
Rat Cell lysate - nuclear (Hippocampal primary Neurons)
Specification
Hippocampal primary Neurons
Negative control
normal Rabbit IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehide 1%
Positive control
BDNF Promoter II
Read More

Abcam user community

Verified customer

投稿 Oct 06 2014

Western blot abreview for Anti-CDYL antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Rat Cell lysate - whole cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Sep 25 2014

Question

Dear Hugh, Thanks for your prompt replay. I want to localize male mice stem cells in female mice tissues. Do you have any other suggestion? If I used this Ab would I be able to differ from the two transcripts? Thanks again. Regards,

Read More

Abcam community

Verified customer

Asked on Sep 29 2005

Answer

Thank you for your reply. Unfortunately, I am not an expert in this field and I have not been able to find any publications relating to the expression profiles in mice stem cells. I am not aware of a particular protein or antibody that would recognise a target uniquely expressed from the Y chromosome in stem cells at least. The majority of genes expressed from the Y chromosome also have X homologs e.g. Smcy and its X homolog Smcx. However, we do have an Sry antibody which is the sex determing factor although I am under the impression that it is uniquely expressed in testes. I would think that the best way to identify male from female cells would be to perform DNA FISH using a commercially available kit and probing X and Y loci. e.g. Males would have green and red foci whereas females would have two green foci. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

Read More

Abcam Scientific Support

Answered on Sep 30 2005

Question

Is it possible to get the peptide sequence for this antibody? And also I would like to know if this protein is specific for the chromosome Y. I am looking for a specific marker to localize male stem cells transplanted into female mice.

Read More

Abcam community

Verified customer

Asked on Sep 28 2005

Answer

Thank you for your enquiry. The peptide sequence used to raise this antibody was a synthetic peptide conjugated to KLH derived from within residues 301-314 of Mouse CDYL, corresponding to sequence TGVTAGKRKFIDDR. In reponse to your question it will depend on whether you wish to localize male human or mouse stem cells transplanted into female mice. Human CDYL is expressed from the Y chromosome but according to http://www.pnas.org/cgi/content/abstract/99/13/8707, murine CDYL is autosomal. I have also found out that in mice it is expressed as 2 transcripts: a ubiquitous transcript and a highly expressed testis-specific transcript. Therefore if you wish to use it as a marker it will depend on the level of expression in the ubiquitous transcript. Unfortunately I could not determine whether it's expression was sex specific, such details were not present on the EXPASY database. I hope that this information helps. Please do not hesitate to contact me should you require further assistance.

Read More

Abcam Scientific Support

Answered on Sep 29 2005

Question

I've ordered the ab5188 antibody. I would like to know if it's ok to block the membrane one hour with TBS+5% non-fat dry milk and incubate the membrane with the primary antibody overnight at 4 degrees ?

Read More

Abcam community

Verified customer

Asked on Aug 03 2004

Answer

Thank you for your enquiry. We would suggest blocking the non-specific binding sited with 5% BSA overnight at 4oC. For further information about Western blot application, please take a look at the Protocol on our website

Read More

Abcam Scientific Support

Answered on Aug 05 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
  • スライド
  • タグ抗体・細胞マーカー
  • その他試薬
サービス&サポート
  • サポート
  • よくあるお問い合わせ
  • プロトコール&トラブルシュート
  • ご購入に関して
  • お問い合わせフォーム
  • トレーニング
  • ターゲット別に見る
アブカムについて
  • 企業ウェブサイト
  • 投資家の皆様へ
  • 企業情報
  • 採用情報
  • アブカムについて
イベント
  • 展示会
  • カンファレンス
International websites
  • abcam.com
  • abcam.cn

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
© 1998-2022 Abcam plc. All rights reserved.