Anti-CDYL 抗体 (ab5188)

Reviews (2)Q&A (3)References (10)

Key features and details

  • Rabbit polyclonal to CDYL
  • Suitable for: WB
  • Reacts with: Mouse, African green monkey
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-CDYL antibody
    CDYL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to CDYL

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, African green monkey
    交差が予測される動物種: Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Mouse CDYL aa 300-400 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13740)

  • ポジティブ･コントロール

    • This antibody gave a positive signal in COS7 cells transfected with Human CDYL (Whole Cell Lysate)

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).

ターゲット情報

  • 機能

    Acts as repressor of transcription (By similarity). Has histone acetyltransferase activity, with a preference for histone H4.

  • 組織特異性

    Ubiquitous. Expressed at moderate levels in all tissues examined.

  • 配列類似性

    Contains 1 chromo domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CDY-like antibody
    • CDY-like autosomal antibody
    • Cdyl antibody
    • CDYL: chromodomain protein Y-like antibody
    • CDYL1 antibody
    • CDYL1_HUMAN antibody
    • chromodomain protein Y chromosome-like 6 antibody
    • Chromodomain protein Y like antibody
    • Chromodomain Y-like protein antibody
    • DKFZP586C1622 antibody
    • MGC131936 antibody
    • OTTHUMP00000015984 antibody
    • OTTHUMP00000211185 antibody
    • OTTHUMP00000211186 antibody
    • OTTHUMP00000211189 antibody
    • testis-specific chromodomain Y-like protein antibody
    • Weakly similar to enoyl-CoA hydratase [H.sapiens] antibody
    see all

画像

  • Western blot - Anti-CDYL antibody (ab5188)
    Western blot - Anti-CDYL antibody (ab5188)
    All lanes : Anti-CDYL antibody (ab5188) at 1 µg/ml

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate - Untransfected
    Lane 2 : COS7 CDYL (Human) Transfected Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 60 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes
  • Western blot - Anti-CDYL antibody (ab5188)
    Western blot - Anti-CDYL antibody (ab5188)Cecile Lestrat, Saadi Khochbin lab, Grenoble, France
    All lanes : Anti-CDYL antibody (ab5188) at 1/1000 dilution

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 2 : COS7 cells transfected with HA-CDYL-FLAG plasmid

    Predicted band size: 66 kDa
    Observed band size: 83 kDa why is the actual band size different from the predicted?



    Rabbit polyclonal to CDYL (ab5188) at 1/1000.

    Left lane (-) COS7 (monkey) cells.
    Right lane (+) COS7 cells transfected with HA-CDYL-FLAG plasmid.

参考文献 (10)

ab5188 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5188 は 10 報の論文で使用されています。

  • Qiu Z  et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
  • Wang J  et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
  • Qi C  et al. Coordinated regulation of dendrite arborization by epigenetic factors CDYL and EZH2. J Neurosci 34:4494-508 (2014). Rat . PubMed: 24671995
  • Escamilla-Del-Arenal M  et al. Cdyl, a new partner of the inactive X chromosome and potential reader of H3K27me3 and H3K9me2. Mol Cell Biol 33:5005-20 (2013). WB, ICC/IF . PubMed: 24144980
  • Bantscheff M  et al. Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nat Biotechnol : (2011). PubMed: 21258344
  • Sass S  et al. MicroRNAs coordinately regulate protein complexes. BMC Syst Biol 5:136 (2011). WB ; Human . PubMed: 21867514
  • Zhang Y  et al. Corepressor Protein CDYL Functions as a Molecular Bridge between Polycomb Repressor Complex 2 and Repressive Chromatin Mark Trimethylated Histone Lysine 27. J Biol Chem 286:42414-25 (2011). WB, ChIP, ChIP-sequencing ; Human . PubMed: 22009739
  • Vermeulen M  et al. Quantitative Interaction Proteomics and Genome-wide Profiling of Epigenetic Histone Marks and Their Readers. Cell 142:967-80 (2010). WB ; Human . PubMed: 20850016
  • Franz H  et al. Multimerization and H3K9me3 Binding Are Required for CDYL1b Heterochromatin Association. J Biol Chem 284:35049-59 (2009). WB ; Human . PubMed: 19808672
  • Kuppuswamy M  et al. Role of the PLDLS-binding cleft region of CtBP1 in recruitment of core and auxiliary components of the corepressor complex. Mol Cell Biol 28:269-81 (2008). WB . PubMed: 17967884

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ChIP abreview for Anti-CDYL antibody - ChIP Grade

 Average
Abreviews
abreview image
Application
ChIP
Detection step
Real-time PCR
Sample
Rat Cell lysate - nuclear (Hippocampal primary Neurons)
Specification
Hippocampal primary Neurons
Negative control
normal Rabbit IgG
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehide 1%
Positive control
BDNF Promoter II
Read More

Abcam user community

Verified customer

投稿 Oct 06 2014

Western blot abreview for Anti-CDYL antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Rat Cell lysate - whole cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

投稿 Sep 25 2014

Question

Dear Hugh, Thanks for your prompt replay. I want to localize male mice stem cells in female mice tissues. Do you have any other suggestion? If I used this Ab would I be able to differ from the two transcripts? Thanks again. Regards,

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Answer

Thank you for your reply. Unfortunately, I am not an expert in this field and I have not been able to find any publications relating to the expression profiles in mice stem cells. I am not aware of a particular protein or antibody that would recognise a target uniquely expressed from the Y chromosome in stem cells at least. The majority of genes expressed from the Y chromosome also have X homologs e.g. Smcy and its X homolog Smcx. However, we do have an Sry antibody which is the sex determing factor although I am under the impression that it is uniquely expressed in testes. I would think that the best way to identify male from female cells would be to perform DNA FISH using a commercially available kit and probing X and Y loci. e.g. Males would have green and red foci whereas females would have two green foci. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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Question

Is it possible to get the peptide sequence for this antibody? And also I would like to know if this protein is specific for the chromosome Y. I am looking for a specific marker to localize male stem cells transplanted into female mice.

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Answer

Thank you for your enquiry. The peptide sequence used to raise this antibody was a synthetic peptide conjugated to KLH derived from within residues 301-314 of Mouse CDYL, corresponding to sequence TGVTAGKRKFIDDR. In reponse to your question it will depend on whether you wish to localize male human or mouse stem cells transplanted into female mice. Human CDYL is expressed from the Y chromosome but according to http://www.pnas.org/cgi/content/abstract/99/13/8707, murine CDYL is autosomal. I have also found out that in mice it is expressed as 2 transcripts: a ubiquitous transcript and a highly expressed testis-specific transcript. Therefore if you wish to use it as a marker it will depend on the level of expression in the ubiquitous transcript. Unfortunately I could not determine whether it's expression was sex specific, such details were not present on the EXPASY database. I hope that this information helps. Please do not hesitate to contact me should you require further assistance.

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Question

I've ordered the ab5188 antibody. I would like to know if it's ok to block the membrane one hour with TBS+5% non-fat dry milk and incubate the membrane with the primary antibody overnight at 4 degrees ?

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Answer

Thank you for your enquiry. We would suggest blocking the non-specific binding sited with 5% BSA overnight at 4oC. For further information about Western blot application, please take a look at the Protocol on our website

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