Anti-CDX2 抗体 [EPR2764Y] (ab76541)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2764Y] to CDX2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CDX2 antibody [EPR2764Y]
CDX2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2764Y] to CDX2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt (Intra) or ICC/IF -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit -
免疫原
Synthetic peptide within Human CDX2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q99626 -
ポジティブ・コントロール
- WB: Caco-2, HT-29, human, mouse and rat colon cancer and human small intestine lysates. IHC-P: Human colon, gastric adenocarcinoma, colonic adenocarcinoma, intestinal tissues; Rat and mouse colon tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
解離定数(KD 値)
KD = 1.00 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2764Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76541の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
For unpurified use at 1/1000 - 1/5000 |
IHC-P | (4) |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500 |
特記事項 |
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WB
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). For unpurified use at 1/1000 - 1/5000 |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500 |
ターゲット情報
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機能
Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. -
配列類似性
Belongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain. -
翻訳後修飾
Phosphorylation of Ser-60 mediates the transactivation capacity. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1045 Human
- Entrez Gene: 12591 Mouse
- Entrez Gene: 66019 Rat
- Omim: 600297 Human
- SwissProt: Q99626 Human
- SwissProt: P43241 Mouse
- Unigene: 174249 Human
- Unigene: 20358 Mouse
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別名
- Caudal type homeo box 2 antibody
- Caudal type homeo box transcription factor 2 antibody
- Caudal type homeobox 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/10000 dilution (Purified)
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human colon cancer lysates
Lane 3 : Rat colon lysates
Lane 4 : Mouse colon lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
Lane 1 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilution (Unpurified)
Lanes 2-3 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilution (Unpurified)
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) lysate
Lane 2 : Colon cancer lysate
Lane 3 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Unpurified ab76541 staining CDX2 in human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Unpurified ab76541 showing positive staining in normal colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Unpurified ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Unpurified ab76541 showing positive staining in colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Unpurified ab76541 showing negative staining in skeletal muscle tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Unpurified ab76541 showing negative staining in normal kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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Unpurified ab76541 showing negative staining in normal brain tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (110)
ab76541 は 110 報の論文で使用されています。
- Calder MD et al. Effects of palmitic acid on localization of embryo cell fate and blastocyst formation gene products. Reproduction 163:133-143 (2022). PubMed: 35038315
- Saari J et al. Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35163236
- Shi M et al. Pasteurized Akkermansia muciniphila Ameliorate the LPS-Induced Intestinal Barrier Dysfunction via Modulating AMPK and NF-κB through TLR2 in Caco-2 Cells. Nutrients 14:N/A (2022). PubMed: 35215413
- Engel RM et al. Modeling colorectal cancer: A bio-resource of 50 patient-derived organoid lines. J Gastroenterol Hepatol 37:898-907 (2022). PubMed: 35244298
- Nahaboo W et al. Keratin filaments mediate the expansion of extra-embryonic membranes in the post-gastrulation mouse embryo. EMBO J 41:e108747 (2022). PubMed: 35266581