The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Belongs to the Caudal homeobox family. Contains 1 homeobox DNA-binding domain.
Phosphorylation of Ser-60 mediates the transactivation capacity.
Caudal type homeo box transcription factor 2 antibody
Caudal type homeobox 2 antibody
Caudal type homeobox protein 2 antibody
Caudal type homeobox transcription factor 2 antibody
Caudal-type homeobox protein 2 antibody
CDX 2 antibody
CDX 3 antibody
Homeobox protein CDX 2 antibody
Homeobox protein CDX-2 antibody
Western blot - Anti-CDX2 antibody (ab48009)
Anti-CDX2 antibody (ab48009) at 1 µg/ml + Human Colon lysate (35µg protein in RIPA buffer).
Predicted band size: 34 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Approx 35kDa band observed in Human Colon lysates (calculated MW of 33.5kDa according to NP_001256.1). Recommended concentration: 1-3µg/ml. The observed 35kDa band was successfully blocked by incubation with the immunizing peptide.