Anti-CDw75 抗体 [LN-1] - BSA and Azide free (ab269786)
Key features and details
- Mouse monoclonal [LN-1] to CDw75 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-CDw75 antibody [LN-1] - BSA and Azide free
CDw75 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [LN-1] to CDw75 - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to CDw75. (Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes).
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ポジティブ・コントロール
- IHC-P: Human spleen tissue.Flow Cytometry: Human lymphocytes.
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特記事項
ab269786 is a carrier free version of ab233965.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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特記事項(精製)
Purified from bioreactor concentrate by PEG precipitation. -
ポリ/モノ
モノクローナル -
クローン名
LN-1 -
アイソタイプ
IgM -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab269786の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.Incubate with primary antibody for 30 minutes at RT.
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Flow Cyt |
Use 0.5-1µg for 10 cells.
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特記事項 |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.Incubate with primary antibody for 30 minutes at RT.
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Flow Cyt
Use 0.5-1µg for 10 cells. |
ターゲット情報
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関連性
CDw75 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. -
細胞内局在
Golgi Apparatus; Golgi stack; Golgi stack membrane; single-pass type II membrane protein. Membrane-bound form in trans cisternae of Golgi. Secreted protein; body fluid. -
参照データベース
- Entrez Gene: 6480 Human
- Omim: 109675 Human
- SwissProt: P15907 Human
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別名
- Alpha 2 6 ST antibody
- B cell antigen CD75 antibody
- Beta galactoside alpha 2 6 sialyltransferase antibody
see all
画像
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Flow cytometric analysis of human lymphocytes labeling CDw75 with ab233965 at 1 µg/106 cells (red), compared to an isotype control (green). PPI negative population analyzed. Surface staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233965).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab269786 は論文での使用が確認できていません。