Anti-CDNF 抗体 [EPR22334-99] (ab253238)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22334-99] to CDNF
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CDNF antibody [EPR22334-99]
CDNF 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22334-99] to CDNF -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Recombinant Human CDNF Protein (aa25-187). Rat and human skeletal muscle tissue lysate. Human testis, liver, brain and kidney tissue lysate. IP: Human skeletal muscle tissue and testis lysate..
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22334-99 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab253238の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/30.
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WB |
1/1000. Predicted molecular weight: 21 kDa.
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特記事項 |
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IP
1/30. |
WB
1/1000. Predicted molecular weight: 21 kDa. |
ターゲット情報
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機能
Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra. -
組織特異性
Widely expressed in neuronal and non-neuronal tissues. In the brain, highest levels in the optic nerve and corpus callosum. -
配列類似性
Belongs to the ARMET family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 441549 Human
- Entrez Gene: 227526 Mouse
- Entrez Gene: 361276 Rat
- Omim: 611233 Human
- SwissProt: Q49AH0 Human
- SwissProt: Q8CC36 Mouse
- SwissProt: P0C5I0 Rat
- Unigene: 559067 Human
see all -
別名
- Arginine rich mutated in early stage tumors like 1 antibody
- ARMET L1 antibody
- ARMET like protein 1 antibody
see all
画像
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CDNF was immunoprecipitated from 0.35 mg human testis tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.
Lane 1: Human testis tissue lysate 10 µg (input)
Lane 2: ab253238 IP in human testis tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human testis tissue lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
CDNF was immunoprecipitated from 0.35 mg human skeletal muscle tissue lysate with ab253238 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab253238 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/1000 dilution.
Lane 1: Human skeletal muscle tissue lysate 10 µg (input)
Lane 2: ab253238 IP in human skeletal muscle tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab253238 in human skeletal muscle lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
All lanes : Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Human kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 21 kDa
Exposure time: 3 minutesBlocking/diluting buffer and concentration: 5% NFDM/TBST.
Kidney and brain are CDNF low expressing tissues (PMID:17611540).
This blot was developed using a higher sensitivity ECL substrate.
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All lanes : Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution
Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle lysate
Lane 3 : Human skeletal muscle lysate
Lane 4 : Human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1 and 2: 37 seconds; Lanes 3 and 4: 3 minutes.
Blocking/diluting buffer and concentration: 5% NFDM/TBST
The expression profile and molecular weight observed are consistent with literature (PMID: 17611540).
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Anti-CDNF antibody [EPR22334-99] (ab253238) at 1/1000 dilution + Recombinant Human CDNF Protein (aa25-187) 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab253238 は論文での使用が確認できていません。