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  1. Link

    cdkn2ap19arf-antibody-ab80.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors Ink4
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Anti-CDKN2A/p19ARF 抗体 (ab80)

  • Datasheet
Reviews (14)Q&A (24)References (207)

Product price, shipping and contact information

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Abpromise

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Western blot - Anti-CDKN2A/p19ARF antibody (ab80)
  • Immunocytochemistry - Anti-CDKN2A/p19ARF antibody (ab80)

Key features and details

  • Rabbit polyclonal to CDKN2A/p19ARF
  • Suitable for: ICC, WB
  • Reacts with: Mouse
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

このターゲットにつきましては、リコンビナント・ウサギ・モノクローナル抗体も販売しています

製品の概要

  • 製品名

    Anti-CDKN2A/p19ARF antibody
    CDKN2A/p19ARF 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to CDKN2A/p19ARF
  • 由来種

    Rabbit
  • 特異性

    On Western blot the antibody reveals IVT murine p19ARF and a band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs.
  • アプリケーション

    適用あり: ICC, WBmore details
  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse CDKN2A/p19ARF aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab2659)

  • 特記事項

    A monoclonal alternative to this target is available (ab26696).

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    The serum has been purified on a peptide-NHS-activated Sepharose column. Specific antibodies have been eluted with 100 mM glycine pH 2.5. The purified antibodies have been dialysed against PBS pH 7.4.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Ink4
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Ink4
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • INK4a/Arf

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

Our Abpromise guarantee covers the use of ab80 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 1 µg/ml. Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle).
WB 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples.

ターゲット情報

  • 関連性

    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • 細胞内局在

    Nuclear
  • 参照データベース

    • Entrez Gene: 12578 Mouse
    • SwissProt: Q64364 Mouse
    • Unigene: 4733 Mouse
    • 別名

      • ARF antibody
      • CDKN2A antibody
      • Cyclin dependent kinase inhibitor 2A antibody
      • INK4 antibody
      • MTS1 antibody
      • P19 antibody
      see all

    画像

    • Western blot - Anti-CDKN2A/p19ARF antibody (ab80)
      Western blot - Anti-CDKN2A/p19ARF antibody (ab80)This image is courtesy of Charles Sherr
      All lanes : Anti-CDKN2A/p19ARF antibody (ab80) at 1 µg/ml

      Lanes 1-2 : mouse embryonic fibroblast total cell extract.
      Lane 3 : mouse embryonic fibroblast total cell extract, with the p19ARF gene knocked out as a negative control.

      Lysates/proteins at 30 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 17 kDa



      Western blot of primary Mouse Embryo Fibroplasts (MEFs) using ab80 at 1 µg/ml.  (30 µg of protein from total cell extracts per lane.)

    • Immunocytochemistry - Anti-CDKN2A/p19ARF antibody (ab80)
      Immunocytochemistry - Anti-CDKN2A/p19ARF antibody (ab80)Pictures were generously provided by Dr. David Bertwistle

      ab80 antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+).

      Cells grown on coverslips were fixed with a 1:1 mixture of methanol:acetone at -20C for 10 mins. After drying, rehydrating and blocking, the co

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (207)

    ab80 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab80 は 207 報の論文で使用されています。

    • Kotowski K  et al. 3PO as a Selective Inhibitor of 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 in A375 Human Melanoma Cells. Anticancer Res 40:2613-2625 (2020). PubMed: 32366406
    • Mikawa R  et al. p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction. Biomolecules 10:N/A (2020). PubMed: 32192082
    • Alameda JP  et al. Premature aging and cancer development in transgenic mice lacking functional CYLD. Aging (Albany NY) 11:127-159 (2019). PubMed: 30631004
    • Hay J  et al. Collaboration of MYC and RUNX2 in lymphoma simulates T-cell receptor signaling and attenuates p53 pathway activity. J Cell Biochem 120:18332-18345 (2019). PubMed: 31257681
    • Benkafadar N  et al. ROS-Induced Activation of DNA Damage Responses Drives Senescence-Like State in Postmitotic Cochlear Cells: Implication for Hearing Preservation. Mol Neurobiol N/A:N/A (2019). PubMed: 30693443
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 38 Abreviews or Q&A

    Western blot abreview for Anti-CDKN2A/p19ARF antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Liver)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (15%)
    Loading amount
    30 µg
    Treatment
    200µM Palmitate
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 08 2020

    Western blot abreview for Anti-CDKN2A/p19ARF antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (lung cancer)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    lung cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 20 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CDKN2A/p19ARF antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (HF)
    Permeabilization
    Yes - PBS-TWEEN 0.05%
    Specification
    HF
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 23 2018

    IHC - Wholemount

    Average
    Abreviews
    Abreviews
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (inner ear tissue)
    Specification
    inner ear tissue
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 11 2013

    Western blot abreview for Anti-CDKN2A/p19ARF antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse whole cell lysate)
    Loading amount
    30 µg
    Specification
    mouse whole cell lysate
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 05 2013

    Question


    Inquiry: Hi, I am having trouble visualizing p19ARF on a WB. I have a band at ˜30 KDa, but none near 19 KDa. I loaded 20ug mouse thymus lysate. A 12% gel was run and then I did a wet transfer onto a nitrocellulose membrane for 2 hours. The WB was blocked for 1h in 5% milk at RT. I incubated with the primary antibody at 4C O/N in 5% milk (1:1000 dilution) and revealed with blot with anti-Rabbit-HRP antibodies which work for other samples. All washes were done with 0.1% TBS-T. Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 08 2012

    Answer

    Thank you for contacting us. Depending on the gel, samples, and buffer, the bands can appear 10 kD from the "predicted" size on your gel. Since the concentration of your antibody is 0.6 mg/ml and we suggest using 1 ug/ml on your blot, you could try using 1/500 primary overnight to see if any other bands appear. You could also use the blocking peptide ab2659 to see if the band on your blot is specifically blocked.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. We do guarantee our products to work as specified on the datasheet and can replace, credit or refund the product within 6 months if you are not satisfied with your results.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 08 2012

    Question

    On the basis of the result of microarray , it is reasonable that the upper band means p19 expression.

    But the positive control (NIH3T3) located on the lower band. Moreover , the band size seems to be a little bit lower than 19 kDa.

    I don’t know which band shows the actual p19. Also, is there any possibility that the location of the band is different between samples and NIH3T3 ?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 30 2012

    Answer

    Thank you for contacting us.

    Based on this image alone it seems like the lower band is your CDKN2A/p19ARF band. It is possible for band size to change slightly due to sample preparation or buffer, however usually the positive control will be a good indicator of where the protein of interest is. One strategy to confirm the band might be to use the blocking peptide (the peptide used as the immunogen) to specifically block the CDKN2A/p19ARF protein. We have this available as ab
    2659.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 30 2012

    Question

    Hello,

    Yesterday, I used your p19ARF antibody(ab80) for my samples. Also I set NIH3T3 whole cell lysate as a positive control, but it didn’t work. (no band.)

    I borrowed this cell line from other lab. After I cultured, I extracted lysate by myself.

    I think there is some possibility that my NIH3T3 had any problems. So, I plan to order your cell lysate and check some mouse embryonic fibroblast cell line; ab7179,ab14874,ab7901,ab3874.

    I’d like to ask you which is the best as positive control for p19ARF antibody? Or is there no difference among them?

    Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2012

    Answer

    Thank you very much for contacting us with your enquiry.

    I'm sorry to hear that there were no bands in Western blot using this antibody. I would expect any of the MEF cell lines to work as a positive control, but I suggest using a whole cell lysate (ab14874 is a nuclear fraction) since that is what we have tested with ab80. What kind of samples are you using other than the MEF lysate?

    If you'd like to send some more details about your protocol ( how much protein was loaded, dilution of antibody, blocking solution, etc) I'd be happy to take a look and see if we have any suggestions to improve the results.

    I look forward to hearing from you. If there's anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Oct 11 2012

    Question

    This antibody is not showing any bands in WB.  I would like a replacement with a new lot.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2011

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab80 from a different lot. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2011

    Question

    Hi Scientific Support,  She called because antibody ab80 is Not working.  They have ordered this many times before, and she wants to know if anyone else has indicated a problem with this lot no. GR7251-1.  She did ask for a replacement.  The order was originally delivered in July, so I informed her that someone would call her back on Monday.  She said that they kept it stored in -20C.  They normally use Mouse, and not the Human version, which is why it was stored for so long.  

    Read More

    Abcam community

    Verified customer

    Asked on Dec 05 2011

    Answer

    My colleague from US Customer Service has passed your message to me. Thank you for your recent call regarding ab80. I am very sorry to hear that you are having problems with this antibody. All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience. We are not aware of any batch-related quality-problem. Could you please check and confirm in what species (sample, cell or tissue types) you have been using this antibody which failed to work? I look forward to hearing from you soon.  

    Read More

    Abcam Scientific Support

    Answered on Dec 05 2011

    1-10 of 38 Abreviews or Q&A

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