Anti-CDKN2A/p19ARF 抗体 (ab80)
Key features and details
- Rabbit polyclonal to CDKN2A/p19ARF
- Suitable for: ICC, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-CDKN2A/p19ARF antibody
CDKN2A/p19ARF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDKN2A/p19ARF -
由来種
Rabbit -
特異性
On Western blot the antibody reveals IVT murine p19ARF and a band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs. -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Synthetic peptide corresponding to Mouse CDKN2A/p19ARF aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab2659) -
特記事項
A monoclonal alternative to this target is available (ab26696).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The serum has been purified on a peptide-NHS-activated Sepharose column. Specific antibodies have been eluted with 100 mM glycine pH 2.5. The purified antibodies have been dialysed against PBS pH 7.4. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use a concentration of 1 µg/ml. Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle).
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| WB | (6) |
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples.
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| 特記事項 |
|---|
|
ICC
Use a concentration of 1 µg/ml. Fix with a 1:1 mixture of methanol:acetone at -20C for 10 mins (see image by Dr. David Bertwistle). |
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WB
1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa). Multiple bands with this antibody have frequently been reported. We believe that this is due to the different modification states of p19ARF. Nuclear extraction may be necessary in some samples. |
ターゲット情報
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関連性
The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 12578 Mouse
- SwissProt: Q64364 Mouse
- Unigene: 4733 Mouse
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別名
- ARF antibody
- CDKN2A antibody
- Cyclin dependent kinase inhibitor 2A antibody
see all
画像
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Western blot - Anti-CDKN2A/p19ARF antibody (ab80)This image is courtesy of Charles SherrAll lanes : Anti-CDKN2A/p19ARF antibody (ab80) at 1 µg/ml
Lanes 1-2 : mouse embryonic fibroblast total cell extract.
Lane 3 : mouse embryonic fibroblast total cell extract, with the p19ARF gene knocked out as a negative control.
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDaWestern blot of primary Mouse Embryo Fibroplasts (MEFs) using ab80 at 1 µg/ml. (30 µg of protein from total cell extracts per lane.)
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Immunocytochemistry - Anti-CDKN2A/p19ARF antibody (ab80)Pictures were generously provided by Dr. David Bertwistleab80 antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+).
Cells grown on coverslips were fixed with a 1:1 mixture of methanol:acetone at -20C for 10 mins. After drying, rehydrating and blocking, the co
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (208)
ab80 は 208 報の論文で使用されています。
- Kotowski K et al. 3PO as a Selective Inhibitor of 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 3 in A375 Human Melanoma Cells. Anticancer Res 40:2613-2625 (2020). PubMed: 32366406
- Mikawa R et al. p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction. Biomolecules 10:N/A (2020). PubMed: 32192082
- Alameda JP et al. Premature aging and cancer development in transgenic mice lacking functional CYLD. Aging (Albany NY) 11:127-159 (2019). PubMed: 30631004
- Hay J et al. Collaboration of MYC and RUNX2 in lymphoma simulates T-cell receptor signaling and attenuates p53 pathway activity. J Cell Biochem 120:18332-18345 (2019). PubMed: 31257681
- Benkafadar N et al. ROS-Induced Activation of DNA Damage Responses Drives Senescence-Like State in Postmitotic Cochlear Cells: Implication for Hearing Preservation. Mol Neurobiol N/A:N/A (2019). PubMed: 30693443