Anti-CDKN1A interacting zinc finger protein 1/CIZ1 抗体 (ab102013)
Key features and details
- Rabbit polyclonal to CDKN1A interacting zinc finger protein 1/CIZ1
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody
CDKN1A interacting zinc finger protein 1/CIZ1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDKN1A interacting zinc finger protein 1/CIZ1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human CDKN1A interacting zinc finger protein 1/CIZ1 aa 200-460.
Database link: NP_036259 -
ポジティブ・コントロール
- A549, HeLa, HepG2, HCT116 IP: HeLa whole cell lysate. IHC-P: ES2 xenograft tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab102013の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 91 kDa.
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IHC-P |
1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 91 kDa. |
IHC-P
1/100 - 1/500. antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH8.0) is recommended. |
ターゲット情報
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機能
May regulate the subcellular localization of CIP/WAF1. -
関連疾患
Defects in CIZ1 may be a cause of adult onset primary cervical dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Cervical dystonia or spasmodic torticollis, the most common form of focal dystonia, is characterized by involuntary contractions of the neck muscles, which produce abnormal posturing of the head upon the trunk. -
配列類似性
Contains 1 matrin-type zinc finger. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 25792 Human
- Omim: 116899 Human
- SwissProt: Q9ULV3 Human
- Unigene: 212395 Human
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別名
- CDKI antibody
- CDKN1A-interacting zinc finger protein 1 antibody
- Cip1-interacting zinc finger protein antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded ES2 xenograft using ab102013 at 1/500 dilution.
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Paraformaldehyde-fixed HeLa(human epithelial cell line from cervix adenocarcinoma) cells labeling CDKN1A interacting zinc finger protein 1/CIZ1 using ab102013 at 1/200 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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CDKN1A interacting zinc finger protein 1/CIZ1 was immunoprecipitated from 1 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 μg of ab102013. Western blot was performed from the immunoprecipitate using ab102013 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 μg (Input).
Lane 2: Control IgG IP in HeLa whole cell lysate.
Lane 3: ab102013 IP in HeLa whole cell lysate.
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Anti-CDKN1A interacting zinc finger protein 1/CIZ1 antibody (ab102013) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 91 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab102013 は 5 報の論文で使用されています。
- Guo M & Zhang X LncRNA MSTO2P promotes colorectal cancer progression through epigenetically silencing CDKN1A mediated by EZH2. World J Surg Oncol 20:95 (2022). PubMed: 35346226
- Shao Y et al. Ubiquitin-like protein FAT10 promotes renal fibrosis by stabilizing USP7 to prolong CHK1-mediated G2/M arrest in renal tubular epithelial cells. Aging (Albany NY) 14:7527-7546 (2022). PubMed: 36152057
- Zhou X et al. CDKN1A-interacting zinc finger protein 1 is a novel biomarker for lung squamous cell carcinoma. Oncol Lett 15:183-188 (2018). WB, IHC-P ; Human . PubMed: 29285191
- Gang X et al. Treatment effect of CDKN1A on rheumatoid arthritis by mediating proliferation and invasion of fibroblast-like synoviocytes cells. Clin Exp Immunol N/A:N/A (2018). PubMed: 29920650
- Wang DQ et al. Ciz1 is a novel predictor of survival in human colon cancer. Exp Biol Med (Maywood) N/A:N/A (2014). PubMed: 24928862