Application
ChIP
Sample
Mouse Cell lysate - whole cell (G1E cell line)
Negative control
IgG ChIP on regions
Specification
G1E cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 30 min of EGS+3 min of formald
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 30 min of EGS+3 min of formald
Positive control
CHD4 ChIP on KIT TSS and TAL1 TSS
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dilution buffer
Additional data
Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback about the use of ab6544 in flow cytometry, an application we have not tested before.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 02 2018