Anti-Cdk6 抗体 (ab151247)
Key features and details
- Rabbit polyclonal to Cdk6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cdk6 antibody
Cdk6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdk6 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow -
免疫原
Recombinant fragment, corresponding to a region within amino acids 35-309 of Human Cdk6 (UniProt Q00534).
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ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human SW480 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151247の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 420558 Chicken
- Entrez Gene: 511754 Cow
- Entrez Gene: 1021 Human
- Entrez Gene: 12571 Mouse
- Entrez Gene: 114483 Rat
- Omim: 603368 Human
- SwissProt: Q00534 Human
- SwissProt: Q64261 Mouse
see all -
別名
- CDK 6 antibody
- CDK6 antibody
- CDK6_HUMAN antibody
see all
画像
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Anti-Cdk6 antibody (ab151247) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PPIH with ab151247 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue labeling Cdk6 with ab151247 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab151247 は 57 報の論文で使用されています。
- Xu X et al. Proteomics analysis reveals the correlation of programmed ROS-autophagy loop and dysregulated G1/S checkpoint with imatinib resistance in chronic myeloid leukemia cells. Proteomics 22:e2100094 (2022). PubMed: 34564948
- Yao J et al. miR-342-3p Suppresses glioblastoma development via targeting CDK6. Acta Biochim Pol 69:393-397 (2022). PubMed: 35616621
- Li D et al. C8orf76 Modulates Ferroptosis in Liver Cancer via Transcriptionally Up-Regulating SLC7A11. Cancers (Basel) 14:N/A (2022). PubMed: 35884471
- Zhai Y et al. Long Non-Coding RNA LINC00313 Accelerates Cervical Carcinoma Progression by miR-4677-3p/CDK6 Axis. Onco Targets Ther 14:2213-2226 (2021). PubMed: 33824592
- Xu Q et al. All-trans retinoic acid controls differentiation, proliferation, and lipolysis in isolated subcutaneous adipocytes from peripartal Holstein cows. J Dairy Sci 104:4999-5008 (2021). PubMed: 33551168