Anti-Cdk6 抗体 [98D] (ab241554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [98D] to Cdk6
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cdk6 antibody [98D]
Cdk6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [98D] to Cdk6 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Mouse Cdk6 aa 1-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q00534 -
ポジティブ・コントロール
- WB: HeLa, Jurkat, K562, RAW 264.7 and PC-12 whole cell lysates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
98D -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab241554の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.518 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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特記事項 |
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WB
Use a concentration of 0.518 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
ターゲット情報
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機能
Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 1021 Human
- Entrez Gene: 12571 Mouse
- Entrez Gene: 114483 Rat
- Omim: 603368 Human
- SwissProt: Q00534 Human
- SwissProt: Q64261 Mouse
- Unigene: 119882 Human
- Unigene: 31672 Mouse
see all -
別名
- CDK 6 antibody
- CDK6 antibody
- CDK6_HUMAN antibody
see all
画像
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All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 1/2000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CDK6 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab241554 observed at 38 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab241554 was shown to react with Cdk6 in HeLa wild-type cells in Western blot with loss of signal observed in CDK6 knockout cell line ab266059 (CDK6 knockout cell lysate ab257088). Wild-type HeLa and CDK6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab241554 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/ml
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 3 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate
Lane 4 : Saos-2 (human osteosarcoma epithelial), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Negative control: PANC-1, Saos-2. (PMID: 8114739).
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All lanes : Anti-Cdk6 antibody [98D] (ab241554) at 0.518 µg/ml
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab241554 は 4 報の論文で使用されています。
- Guo X et al. High Expression of PIGC Predicts Unfavorable Survival in Hepatocellular Carcinoma. J Hepatocell Carcinoma 8:211-222 (2021). PubMed: 33854986
- Wang Y et al. Hsa_circ_0002062 Promotes the Proliferation of Pulmonary Artery Smooth Muscle Cells by Regulating the Hsa-miR-942-5p/CDK6 Signaling Pathway. Front Genet 12:673229 (2021). PubMed: 34322152
- Sheng W et al. Upregulation of Linc00284 Promotes Lung Cancer Progression by Regulating the miR-205-3p/c-Met Axis. Front Genet 12:694571 (2021). PubMed: 34616424
- Wu Q et al. Salvianolic Acid Alleviated Blood-Brain Barrier Permeability in Spontaneously Hypertensive Rats by Inhibiting Apoptosis in Pericytes via P53 and the Ras/Raf/MEK/ERK Pathway. Drug Des Devel Ther 14:1523-1534 (2020). PubMed: 32368011