Anti-CDK5RAP3 抗体 [EPR11294(B)] (ab157203)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11294(B)] to CDK5RAP3
- Suitable for: WB, IP, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-CDK5RAP3 antibody [EPR11294(B)]
CDK5RAP3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11294(B)] to CDK5RAP3 -
由来種
Rabbit -
特異性
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.
-
アプリケーション
適用あり: WB, IP, IHC-P, Flow Cyt (Intra)more details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human CDK5RAP3 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q96JB5 -
ポジティブ・コントロール
- WB: Jurkat, HeLa, A431, CTLL-2, EL4, H9 and 293T cell lysates. IHC-P: Human pancreas and stomach tissue. Flow Cyt (intra): HEK-293T cells. IP: CDK5RAP3 IP in HEK-293T cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11294(B) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157203の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
|
|
IP |
1/10 - 1/100.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1. -
組織特異性
Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta. -
配列類似性
Belongs to the CDK5RAP3 family. -
翻訳後修飾
Phosphorylated in vitro by CDK5. - Information by UniProt
-
参照データベース
- Entrez Gene: 80279 Human
- Entrez Gene: 80280 Mouse
- Omim: 608202 Human
- SwissProt: Q96JB5 Human
- SwissProt: Q99LM2 Mouse
- Unigene: 20157 Human
- Unigene: 28297 Mouse
-
別名
- C53 antibody
- CDK5 activator binding protein C53 antibody
- CDK5 activator-binding protein C53 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CDK5RAP3 with purified ab157203 at 1/50 dilution (2.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/2000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution (Purified)
Lane 1 : CTLL-2 (Mouse T lymphocyte) whole cell lysates
Lane 2 : EL4 (Mouse lymphoma T lymphocyte) whole cell lysates
Lane 3 : H9 (Human lymphoma T lymphocyte) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
ab157203 (purified) at 1/20 dilution (1ug) immunoprecipitating CDK5RAP3 in HEK-293 whole cell lysate. HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab157203 & HEK-293 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab157203 in HEK-293 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Intracellular Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling CDK5RAP3 with purified ab157203 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue, labeling CDK5RAP3 using ab157203 (unpurified) at a 1/50 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Western blot analysis of Immunoprecipitation pellet from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate showing CDK5RAP3, using ab157203 (unpurified) at 1/10 dilution.
-
All lanes : Anti-CDK5RAP3 antibody [EPR11294(B)] (ab157203) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa -
Intracellular Flow Cytometry analysis of permeabilized HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling CDK5RAP3 (red), using ab157203 (unpurified) at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab157203 は 6 報の論文で使用されています。
- Hu X et al. Ubiquitin fold modifier 1 activates NF-?B pathway by down-regulating LZAP expression in the macrophage of diabetic mouse model. Biosci Rep 40:N/A (2020). PubMed: 31829413
- Yang R et al. CDK5RAP3, a UFL1 substrate adaptor, is crucial for liver development. Development 146:N/A (2019). PubMed: 30635284
- Chen QY et al. CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer. J Cancer 10:4488-4498 (2019). PubMed: 31528213
- Lin JX et al. CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1a/VEGFA signaling. Cancer Cell Int 19:282 (2019). PubMed: 31728130
- Zheng CH et al. CDK5RAP3 suppresses Wnt/ß-catenin signaling by inhibiting AKT phosphorylation in gastric cancer. J Exp Clin Cancer Res 37:59 (2018). PubMed: 29540196
- Lin JX et al. Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer. World J Gastroenterol 24:3898-3907 (2018). PubMed: 30228783