Anti-Cdk2 抗体 [SP80] (ab101682)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP80] to CDK2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cdk2 antibody [SP80]
CDK2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP80] to CDK2 -
由来種
Rabbit -
特異性
Based on the immunogen sequence, we do not expect the antibody to cross-react with other CDK family members. No cross-reactivity testing has been performed.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Cdk2 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P24941 -
ポジティブ・コントロール
- IHC-P: Human tonsil, mouse and rat spleen
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP80 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101682の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50. Predicted molecular weight: 34 kDa. for 1 hour at RT.
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IHC-P |
1/100. Incubate for 30 minutes at RT. Antigen retrieval is recommended: boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min.
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特記事項 |
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WB
1/50. Predicted molecular weight: 34 kDa. for 1 hour at RT. |
IHC-P
1/100. Incubate for 30 minutes at RT. Antigen retrieval is recommended: boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. |
ターゲット情報
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機能
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
- SwissProt: Q63699 Rat
- Unigene: 19192 Human
see all -
別名
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- CDC28 antibody
see all
画像
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All lanes : Anti-Cdk2 antibody [SP80] (ab101682) at 1/200 dilution
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : CDK2 knockout MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaWestern blot: Anti-CDK2 antibody [SP80] (ab101682) staining at 1/200 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab101682 was shown to bind specifically to CDK2. A band was observed at 34 kDa in wild-type MCF7 cell lysates with no signal observed at this size in CDK2 knockout cell line ab282628. To generate this image, wild-type and CDK2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Cdk2 with ab101682 at 1/50 dilution (2.20 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101682 for 30 mins at room temperature. -
Anti-Cdk2 antibody [SP80] (ab101682) at 1/100 dilution + lysate prepared from HeLa cells
Predicted band size: 34 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling Cdk2 with ab101682 at 1/50 dilution (2.20 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101682 for 30 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling Cdk2 with ab101682 at 1/50 dilution (2.20 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101682 for 30 mins at room temperature. -
Immunohistochemical analysis of Cdk2 expression in formalin-fixed, paraffin-embedded Human tonsil using ab101682 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab101682 は 4 報の論文で使用されています。
- Chen C et al. ASF1b is a novel prognostic predictor associated with cell cycle signaling pathway in gastric cancer. J Cancer 13:1985-2000 (2022). PubMed: 35399734
- Zhang Y et al. LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940. J Hepatocell Carcinoma 8:333-348 (2021). PubMed: 33977095
- Ji W et al. Combined Androgen receptor blockade overcomes the resistance of breast cancer cells to palbociclib. Int J Biol Sci 15:522-532 (2019). PubMed: 30745839
- Qi H et al. Activation of AMPK Attenuated Cardiac Fibrosis by Inhibiting CDK2 via p21/p27 and miR-29 Family Pathways in Rats. Mol Ther Nucleic Acids 8:277-290 (2017). PubMed: 28918029