Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (osteosarcoma cells)
Specification
osteosarcoma cells
Treatment
lane 1 control, lane 2 siRNA for CDK1, 48h
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 2% BSA TBS-T
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbitt
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 34 kDa
Exposure
2 second(s)
Negative control
lane 2 - siRNA for CDK1
Positive control
lane 1 - control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sonia Rocha
Verified customer
投稿 Oct 13 2014