Name: Anti-CDK1 antibody [EPR165]
Brand: RabMAb
SKU: ab133327
Rating: 5 (3 reviews)

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Western blot - Anti-CDK1 antibody [EPR165] (ab133327)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR165] to CDK1
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-P
Reacts with: Human

Carrier Free

製品名

Anti-CDK1 antibody [EPR165]
CDK1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR165] to CDK1
由来種

Rabbit
特異性

We have preliminary internal testing data to indicate this antibody doesn’t react with CDK2, CDK3 and CDK5. Please contact us for more information.
アプリケーション

適用あり: Flow Cyt (Intra), ICC/IF, WB, IP, IHC-Pmore details
種交差性

交差種: Human
免疫原

Synthetic peptide within Human CDK1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P06493
ポジティブ･コントロール
- WB: HeLa, Saos-2, Namalwa, and Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue. IP: HeLa. Flow Cyt: Jurkat cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR165
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-CDK1 antibody [EPR165] - BSA and Azide free (ab224269)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab7899)
Recombinant Protein
- Recombinant Human CDK1 protein (ab187447)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab133327の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF	(1)	1/100 - 1/250.
WB	(2)	1/10000 - 1/50000. Predicted molecular weight: 34 kDa.
IP		1/10 - 1/100.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

特記事項
Flow Cyt (Intra) 1/130. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
WB 1/10000 - 1/50000. Predicted molecular weight: 34 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

機能

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
組織特異性

Isoform 2 is found in breast cancer tissues.
配列類似性

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
細胞内局在

Nucleus.
Target information above from: UniProt accession P06493 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
製品の状態

CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
別名
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
- CDK 1 antibody
- CDK1 antibody
- CDK1_HUMAN antibody
- CDKN1 antibody
- CELL CYCLE CONTROLLER CDC2 antibody
- Cell division control protein 2 antibody
- Cell division control protein 2 homolog antibody
- Cell division cycle 2 G1 to S and G2 to M antibody
- Cell division protein kinase 1 antibody
- Cell Divsion Cycle 2 Protein antibody
- Cyclin Dependent Kinase 1 antibody
- Cyclin-dependent kinase 1 antibody
- DKFZp686L20222 antibody
- MGC111195 antibody
- p34 Cdk1 antibody
- p34 protein kinase antibody
- P34CDC2 antibody
see all

Western blot - Anti-CDK1 antibody [EPR165] (ab133327)

All lanes : Anti-CDK1 antibody [EPR165] (ab133327) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : Namalwa cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CDK1 antibody [EPR165] (ab133327)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling CDK1 with ab133327 at 1/500 dilution (0.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor^® 488) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). DAPI nuclear counterstain. Confocal image showing strong nuclear and weakly cytoplasmic staining on HeLa cell line.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [EPR165] (ab133327)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling CDK1 with ab133327 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-CDK1 antibody [EPR165] (ab133327)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CDK1 with purified ab133327 at 1/130 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Immunoprecipitation - Anti-CDK1 antibody [EPR165] (ab133327)

CDK1 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with 133327 at 1/20 dilution (2µg) . VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg

Lane 2: ab133327 IP in HeLa whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133327 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-CDK1 antibody [EPR165] (ab133327)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

ab133327 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab133327 は 44 報の論文で使用されています。

Yang T et al. Punicalin Alleviates OGD/R-Triggered Cell Injury via TGF-ß-Mediated Oxidative Stress and Cell Cycle in Neuroblastoma Cells SH-SY5Y. Evid Based Complement Alternat Med 2021:6671282 (2021). PubMed: 33628309
Lv C et al. High Iodine Induces the Proliferation of Papillary and Anaplastic Thyroid Cancer Cells via AKT/Wee1/CDK1 Axis. Front Oncol 11:622085 (2021). PubMed: 33796458
Zhao J et al. lncRNA LIFR-AS1 inhibits gastric carcinoma cell proliferation, migration and invasion by sponging miR-4698. Mol Med Rep 23:N/A (2021). PubMed: 33355363
Tian L et al. Integrated Protein-Protein Interaction and Weighted Gene Co-expression Network Analysis Uncover Three Key Genes in Hepatoblastoma. Front Cell Dev Biol 9:631982 (2021). PubMed: 33718368
Zhou Z et al. lncRNA SNHG4 modulates colorectal cancer cell cycle and cell proliferation through regulating miR-590-3p/CDK1 axis. Aging (Albany NY) 13:9838-9858 (2021). PubMed: 33744866

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (MCF10A cells)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

MCF10A cells

Blocking step

10% FBS, 1% BSA, 0.1% Triton X-100 in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 11% · Temperature: 22°C

Fixative

Formaldehyde

Abcam user community

Verified customer

投稿 Mar 08 2022

Application

Western blot

Sample

Mouse Cell lysate - whole cell (MEF, HEK and PC3)

Gel Running Conditions

Reduced Denaturing

Loading amount

10 µg

Specification

MEF, HEK and PC3

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

投稿 May 19 2017

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Cell lysate - whole cell (osteosarcoma cells)

Specification

osteosarcoma cells

Treatment

lane 1 control, lane 2 siRNA for CDK1, 48h

Blocking step

Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C

DR. Sonia Rocha

Verified customer

投稿 Oct 13 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

製品の状態

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (44)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-CDK1 antibody [EPR165]

Western blot abreview for Anti-CDK1 antibody [EPR165]

Western blot abreview for Anti-CDK1 antibody [EPR165]