Anti-CDCP1 抗体 (ab1377)

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製品の概要

  • 製品名

    Anti-CDCP1 antibody
    CDCP1 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to CDCP1

  • 由来種

    Goat

  • 特異性

    This antibody is expected to recognize isoform 1 (NP_073753.3) only. Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WB, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human Colon extracts.

  • 特記事項

    GenBank Accession Number - NP_073753

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 16823897
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
IP Use at an assay dependent dilution. PubMed: 17785447

ターゲット情報

  • 機能

    May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.

  • 組織特異性

    Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.

  • 配列類似性

    Contains 1 CUB domain.

  • 翻訳後修飾

    Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
    N-glycosylated.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.

  • 細胞内局在

    Secreted and Cell membrane. Shedding may also lead to a soluble peptide.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 9030022E12Rik antibody
      • AA409659 antibody
      • CD 318 antibody
      • CD_antigen=CD318 antibody
      • CD318 antibody
      • CD318 antigen antibody
      • CDCP 1 antibody
      • Cdcp1 antibody
      • CDCP1_HUMAN antibody
      • CUB domain containing protein 1 antibody
      • CUB domain-containing protein 1 antibody
      • E030027H19Rik antibody
      • FLJ13772 antibody
      • FLJ22375 antibody
      • FLJ22969 antibody
      • Membrane glycoprotein gp140 antibody
      • MGC31813 antibody
      • OTTHUMP00000164120 antibody
      • OTTHUMP00000209568 antibody
      • RGD1305578 antibody
      • SIMA 135 antibody
      • SIMA135 antibody
      • Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
      • Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
      • Transmembrane and associated with src kinases antibody
      • TRASK antibody
      see all

    画像

    • Western blot - Anti-CDCP1 antibody (ab1377)
      Western blot - Anti-CDCP1 antibody (ab1377)
      ab1377 (1 µg/ml) staining CDCP1 human colon whole cell lysate (35 µg) in RIPA buffer. Incubation was for 1 hour and detection was by chemiluminescence.
    • Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody (ab1377)
      Immunocytochemistry/ Immunofluorescence - Anti-CDCP1 antibody (ab1377)
      ICC/IF image of ab1377 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1377, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCP1 antibody (ab1377)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDCP1 antibody (ab1377)
      IHC image of ab1377 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1377, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献

    This product has been referenced in:

    • Wright HJ  et al. CDCP1 cleavage is necessary for homodimerization-induced migration of triple-negative breast cancer. Oncogene 35:4762-72 (2016). IF . Read more (PubMed: 26876198) »
    • Chiu KL  et al. ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis. Sci Rep 5:16426 (2015). Read more (PubMed: 26553452) »
    See all 12 Publications for this product

    ab1377 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Question

    Csutomer kindly called to inquire about our CDCP1 antibodies, quality control data and use in FACS.

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    Answer

    Thank you for contacting us.

    I have been able to attain immunogen data used to create the product ab118268 for you which has been created using the full length recombinant protein. I am still awaiting further data including any additional quality control or information about used of these products in FACs.



    While we cannot be sure of the region to which these product bind, for UNTESTED species and/or applications, sucgh as FACs with these products, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.



    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    This antibody is a fast track. Can I provide details of image and what "low signal" refers to when tested against Human Liver and Brain lysates.

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    Answer

    Thank you for your enquiry. Further to correspondence with the source of this antibody I have determined that this antibody was tested by western blotting using standard tissues/cells, on this occassion human liver and brain lysates. No bands were observed and the general background was low. It is not known whether this protein is expressed in these cells. I am sorry that I cannot be of more assistance.

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    Question

    I request some information about your anti-gamma tubulin antibodies. I would like to know, in as much detail as possible, about what antigen the antibodies (ab1377, ab11318, ab11320, ab11321) directed against gamma tubulin were raised. Many of the antibodies on the market were raised against peptide 38-53 of human gamma tubulin. Were any of your antibodies raised against a different portion of the gamma tubulin protein, or against a full length gamma tubulin? Are you at liberty to disclose who (which laboratory) produced each antibody originally? Thank you,

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    Answer

    Thank you for your enquiry. Details of the immunogens used for each of the antisera that you are interested in are as follows: I think that you may have mistyped the first antiserum as it is not directed against tubulin. CDCP1 antibody (ab1377) - Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1. Gamma Tubulin antibody - Centrosome Marker (ab11318) - Synthetic peptide: EEFATEGTDRKDVFFY-K, corresponding to amino acids 38-53 of Human gamma Tubulin. Gamma Tubulin antibody - Centrosome Marker (ab11320) - Synthetic peptide: DEYHAATRPDYISWGTQEQ corresponding to amino acids 433-451 of gamma Tubulin. Gamma Tubulin antibody - Centrosome Marker (ab11321) Synthetic peptide: AATRPDYISWGTQEQ corresponding to amino acids 437-451 of Xenopus laevis gamma Tubulin. I hope this information helps, please do not hesitate to contact me should you require further assistance.

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    Question

    Do you have a sample antibody program? I am really tired of buying Abs that don't work. At least you are forward enough to state that it hasn't worked as yet on the tested samples. If you could send me a free trial amount of the antibody and it works, I will order a full aliquot. I am also in a position to test your Ab, as I have monoclonal antibodies to CDCP1 that do work - too well, in fact. My antibodies were made with a peptide sequence that probably contains a sequence conserved in multiple proteins since they are recognizing multiple proteins. Moreover, all protein interactions can be inhibited by immunogen peptide but not an irrelevant peptide. Anyway, your antibody would potentially allow me to define specific CDCP1 interactions, and I would be able to provide you with information on your antibody reactivity. Please let me know if this is a mutually agreeable avenue. Thanks.

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    Answer

    I am afraid that I am unable to offer you a trial sample of this antibody for preliminary testing. The antibody is priced to reflect the fact that it is investigative grade and all the information we have is on the datasheet.

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