• 製品名

  • 製品の詳細

    Goat polyclonal to CDCP1
  • 由来種

  • 特異性

    This antibody is expected to recognize isoform 1 (NP_073753.3) only. Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.
  • アプリケーション

    適用あり: ICC/IF, IHC-P, WB, IPmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1.

  • ポジティブ・コントロール

    • Human Colon extracts.
  • 特記事項

    GenBank Accession Number - NP_073753



Our Abpromise guarantee covers the use of ab1377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 16823897
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 92 kDa).
IP Use at an assay dependent dilution. PubMed: 17785447


  • 機能

    May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
  • 組織特異性

    Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
  • 配列類似性

    Contains 1 CUB domain.
  • 翻訳後修飾

    Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
    A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.
  • 細胞内局在

    Secreted and Cell membrane. Shedding may also lead to a soluble peptide.
  • Information by UniProt
  • 参照データベース

  • 別名

    • 9030022E12Rik antibody
    • AA409659 antibody
    • CD 318 antibody
    • CD_antigen=CD318 antibody
    • CD318 antibody
    • CD318 antigen antibody
    • CDCP 1 antibody
    • Cdcp1 antibody
    • CDCP1_HUMAN antibody
    • CUB domain containing protein 1 antibody
    • CUB domain-containing protein 1 antibody
    • E030027H19Rik antibody
    • FLJ13772 antibody
    • FLJ22375 antibody
    • FLJ22969 antibody
    • Membrane glycoprotein gp140 antibody
    • MGC31813 antibody
    • OTTHUMP00000164120 antibody
    • OTTHUMP00000209568 antibody
    • RGD1305578 antibody
    • SIMA 135 antibody
    • SIMA135 antibody
    • Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
    • Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
    • Transmembrane and associated with src kinases antibody
    • TRASK antibody
    see all


  • ab1377 (1 µg/ml) staining CDCP1 human colon whole cell lysate (35 µg) in RIPA buffer. Incubation was for 1 hour and detection was by chemiluminescence.
  • ICC/IF image of ab1377 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1377, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab1377 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1377, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Wright HJ  et al. CDCP1 cleavage is necessary for homodimerization-induced migration of triple-negative breast cancer. Oncogene 35:4762-72 (2016). IF . Read more (PubMed: 26876198) »
  • Chiu KL  et al. ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis. Sci Rep 5:16426 (2015). Read more (PubMed: 26553452) »
See all 12 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A


Thank you for contacting us.

I have been able to attain immunogen data used to create the product ab118268 for you which has been created using the full length recombinant protein. I am still awaiting further data including any additional quality control or information about used of these products in FACs.

While we cannot be sure of the region to which these product bind, for UNTESTED species and/or applications, sucgh as FACs with these products, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!

Read More


Thank you for your enquiry. Further to correspondence with the source of this antibody I have determined that this antibody was tested by western blotting using standard tissues/cells, on this occassion human liver and brain lysates. No bands were observed and the general background was low. It is not known whether this protein is expressed in these cells. I am sorry that I cannot be of more assistance.

Read More


Thank you for your enquiry. Details of the immunogens used for each of the antisera that you are interested in are as follows: I think that you may have mistyped the first antiserum as it is not directed against tubulin. CDCP1 antibody (ab1377) - Synthetic peptide: PLLNTQEPMEPAE, corresponding to C terminal amino acids 824-836 of Human CDCP1. Gamma Tubulin antibody - Centrosome Marker (ab11318) - Synthetic peptide: EEFATEGTDRKDVFFY-K, corresponding to amino acids 38-53 of Human gamma Tubulin. Gamma Tubulin antibody - Centrosome Marker (ab11320) - Synthetic peptide: DEYHAATRPDYISWGTQEQ corresponding to amino acids 433-451 of gamma Tubulin. Gamma Tubulin antibody - Centrosome Marker (ab11321) Synthetic peptide: AATRPDYISWGTQEQ corresponding to amino acids 437-451 of Xenopus laevis gamma Tubulin. I hope this information helps, please do not hesitate to contact me should you require further assistance.

Read More

For licensing inquiries, please contact partnerships@abcam.com