Anti-CDCA5 抗体 [EPR16331] - C-terminal (ab192237)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CDCA5 antibody [EPR16331] - C-terminal
CDCA5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16331] to CDCA5 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- 293, HeLa, Jurkat and SH-SY5Y cell lysates; Human stomach and human endometrium adenocarcinoma tissues; HeLa and 293 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16331 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab192237の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100.
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WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/100. |
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair. -
配列類似性
Belongs to the sororin family. -
ドメイン
The KEN box is required for the association with the APC/C complex. -
翻訳後修飾
Phosphorylated. Phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A and relieves the inhibition of WAPAL by CDCA5.
Ubiquitinated by the APC/C complex in G1, leading to its degradation. -
細胞内局在
Nucleus. Chromosome. Cytoplasm. Associates with nuclear chromatin from S phase until metaphase and is released in the cytoplasm upon nuclear envelope breakdown. - Information by UniProt
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参照データベース
- Entrez Gene: 113130 Human
- Omim: 609374 Human
- SwissProt: Q96FF9 Human
- Unigene: 434886 Human
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別名
- CDCA5 antibody
- CDCA5_HUMAN antibody
- Cell division cycle associated protein 5 antibody
see all
画像
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All lanes : Anti-CDCA5 antibody [EPR16331] - C-terminal (ab192237) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
Blocking and diluting buffer and concntration: 5% NFDM/TBST
Imaging carried out using iBright CL 1000 Imaging SystemExposure time: Lane 1: 180 seconds, Lane 2: 48 seconds.
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Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling CDCA5 with ab192237 at 1/100 dilution (5μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-CDCA5 at 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/400 dilution. -
Intracellular flow cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling CDCA5with ab192237 at 1/100 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CDCA5 with ab192237 at 1/100 (5 µg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (14)
ab192237 は 14 報の論文で使用されています。
- Huang X et al. Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. Int J Oncol 61:N/A (2022). PubMed: 35642672
- Ji J et al. CDCA5 promotes the progression of prostate cancer by affecting the ERK signalling pathway. Oncol Rep 45:921-932 (2021). PubMed: 33650660
- Zhou C et al. The cohesin stabilizer Sororin drives G2-M transition and spindle assembly in mammalian oocytes. Sci Adv 7:eabg9335 (2021). PubMed: 34559563
- He X et al. USP13 interacts with cohesin and regulates its ubiquitination in human cells. J Biol Chem 296:100194 (2021). PubMed: 33334891
- Luo Z et al. SPOP promotes CDCA5 degradation to regulate prostate cancer progression via the AKT pathway. Neoplasia 23:1037-1047 (2021). PubMed: 34509929