Anti-CDC42 binding protein kinase alpha 抗体 (ab96659)
Key features and details
- Rabbit polyclonal to CDC42 binding protein kinase alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CDC42 binding protein kinase alpha antibody
CDC42 binding protein kinase alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDC42 binding protein kinase alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment, corresponding to a region within amino acids 373-574 of Human CDC42 binding protein kinase alpha isoform 3 (NP_055641).
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ポジティブ・コントロール
- H1299, A549 and HeLa whole cell lysate (ab150035)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96659の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 186 kDa.
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IHC-P |
1/100 - 1/500. Perform heat-mediated antigen retrieval with 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 186 kDa. |
IHC-P
1/100 - 1/500. Perform heat-mediated antigen retrieval with 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) |
ターゲット情報
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機能
May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation. -
組織特異性
Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 CNH domain.
Contains 1 CRIB domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent. - Information by UniProt
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参照データベース
- Entrez Gene: 8476 Human
- Entrez Gene: 226751 Mouse
- Entrez Gene: 114116 Rat
- GenBank: NP_055641 Human
- Omim: 603412 Human
- SwissProt: Q5VT25 Human
- SwissProt: Q3UU96 Mouse
- SwissProt: O54874 Rat
see all -
別名
- A930014J19Rik antibody
- CDC42 binding protein kinase alpha (DMPK like) antibody
- CDC42 binding protein kinase alpha antibody
see all
画像
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Anti-CDC42 binding protein kinase alpha antibody (ab96659) at 1/1500 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 186 kDa
7.5% SDS PAGE -
ab96659 staining CDC42 binding protein kinase alpha in SW480 xenograft at 1/100 dilution by Immunohistochemistry (Formalin/PFA-Fixed Paraffin-embedded sections)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab96659 は 5 報の論文で使用されています。
- Wang F et al. MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells. Anim Nutr 10:319-328 (2022). PubMed: 35891685
- Kurimchak AM et al. Functional proteomics interrogation of the kinome identifies MRCKA as a therapeutic target in high-grade serous ovarian carcinoma. Sci Signal 13:N/A (2020). PubMed: 32071169
- Konopaske GT et al. Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia. Schizophr Res N/A:N/A (2018). PubMed: 29759351
- Unbekandt M et al. Discovery of Potent and Selective MRCK Inhibitors with Therapeutic Effect on Skin Cancer. Cancer Res 78:2096-2114 (2018). PubMed: 29382705
- Zhu KC et al. Afzelin exhibits anti-cancer activity against androgen-sensitive LNCaP and androgen-independent PC-3 prostate cancer cells through the inhibition of LIM domain kinase 1. Oncol Lett 10:2359-2365 (2015). WB ; Human . PubMed: 26622852