Rabbit polyclonal to Cdc37
Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
Synthetic peptide, corresponding to a region within amino acids 1 -50 of Human Cdc37 (Q16543).
Hela, 293T and mpuse NIH3T3 cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8.
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
Use at 2-5 µg/mg of lysate.
Use at an assay dependent concentration.
Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.
Belongs to the CDC37 family.
Constitutively sumoylated by UBE2I.
Information by UniProt
CDC 37 antibody
CDC37 cell division cycle 37 homolog antibody
Western blot - Anti-Cdc37 antibody (ab115166)
All lanes : Anti-Cdc37 antibody (ab115166) at 0.4 µg/ml Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Lane 5 : Mouse NIH3T3 cell lysate at 50 µg Predicted band size: 44 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-Cdc37 antibody (ab115166)
Immunoprecipitation of Cdc37 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdc37 antibody (ab115166); Lane 2-Immunoprecipitation with an anti-Cdc37 antibody that recognizes an downstream epitope ; Lane 3- Immunoprecipitation with IgG control. Anti-Cdc37 antibody (ab115166) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.
has not yet been referenced specifically in any publications.
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