Anti-Cdc25B 抗体 [EPR3459(2)] - BSA and Azide free (ab248020)
製品の概要
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製品名
Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free
Cdc25B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3459(2)] to Cdc25B - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Cdc25B aa 150-250. The exact sequence is proprietary.
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特記事項
ab248020 is the carrier-free version of ab124819 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248020 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3459(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab248020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). | |
| IP | Use at an assay dependent concentration. |
ターゲット情報
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機能
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. -
配列類似性
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
翻訳後修飾
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. -
細胞内局在
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. - Information by UniProt
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参照データベース
- Entrez Gene: 994 Human
- Omim: 116949 Human
- SwissProt: P30305 Human
- Unigene: 153752 Human
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別名
- AI604853 antibody
- Cdc 25B antibody
- Cdc25b antibody
see all
データシートおよび資料
参考文献
ab248020 has not yet been referenced specifically in any publications.
