Anti-Cdc25B 抗体 [EPR3459(2)] - BSA and Azide free (ab248020)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3459(2)] to Cdc25B - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
製品の概要
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製品名
Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free
Cdc25B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3459(2)] to Cdc25B - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Cdc25B aa 150-250. The exact sequence is proprietary.
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特記事項
ab248020 is the carrier-free version of ab124819 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248020 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3459(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab248020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). | |
IP | Use at an assay dependent concentration. |
ターゲット情報
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機能
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. -
配列類似性
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
翻訳後修飾
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. -
細胞内局在
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. - Information by UniProt
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参照データベース
- Entrez Gene: 994 Human
- Omim: 116949 Human
- SwissProt: P30305 Human
- Unigene: 153752 Human
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別名
- AI604853 antibody
- Cdc 25B antibody
- Cdc25b antibody
see all
画像
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All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 0.486 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) cell lysates
Lane 2 : Mouse embryo E17 lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 64 kDa
Observed band size: 64 kDaThis data was developed using ab124819, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaThis data was developed using ab124819, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献 (0)
ab248020 は論文での使用が確認できていません。