Anti-Cdc25B 抗体 [EPR3459(2)] - BSA and Azide free (ab248020)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3459(2)] to Cdc25B - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
製品の概要
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製品名
Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free
Cdc25B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3459(2)] to Cdc25B - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Cdc25B aa 150-250. The exact sequence is proprietary.
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特記事項
ab248020 is the carrier-free version of ab124819 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248020 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3459(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab248020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). | |
| IP | Use at an assay dependent concentration. |
ターゲット情報
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機能
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. -
配列類似性
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
翻訳後修飾
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. -
細胞内局在
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. - Information by UniProt
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参照データベース
- Entrez Gene: 994 Human
- Omim: 116949 Human
- SwissProt: P30305 Human
- Unigene: 153752 Human
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別名
- AI604853 antibody
- Cdc 25B antibody
- Cdc25b antibody
see all
画像
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![Western blot - Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free (ab248020)](https://www.abcam.co.jp/ps/products/248/ab248020/Images/ab248020-399068-anti-cdc25b-antibody-epr3459-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free (ab248020)All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDaThis data was developed using ab124819, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献
ab248020 は論文での使用が確認できていません。
