Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3459(2)] to Cdc25B - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
製品名Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free
Cdc25B 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR3459(2)] to Cdc25B - BSA and Azide free
アプリケーション適用あり: WB, IPmore details
適用なし: Flow Cyt
Synthetic peptide within Human Cdc25B aa 150-250. The exact sequence is proprietary.
ab248020 is the carrier-free version of ab124819 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248020 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).|
|IP||Use at an assay dependent concentration.|
機能Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
配列類似性Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.
翻訳後修飾Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
細胞内局在Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
- Information by UniProt
- AI604853 antibody
- Cdc 25B antibody
- Cdc25b antibody
All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
This data was developed using ab124819, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.