Anti-Cdc25A 抗体 (ab989)

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Key features and details

  • Rabbit polyclonal to Cdc25A
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Cdc25A antibody
    Cdc25A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Cdc25A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Chimpanzee, Orangutan

  • 免疫原

    Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 1 - 4 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).

ターゲット情報

  • 機能

    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

  • 配列類似性

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.

  • ドメイン

    The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.

  • 翻訳後修飾

    Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cdc 25a antibody
      • CDC25A antibody
      • CDC25A2 antibody
      • CDC25A2 CAG isoform antibody
      • Cell division cycle 25 homolog A (S. pombe) antibody
      • Cell division cycle 25A antibody
      • Cell division cycle 25A isoform a antibody
      • Cell division cycle 25A isoform b antibody
      • D9Ertd393e antibody
      • Dual specificity phosphatase Cdc25A antibody
      • M phase inducer phosphatase 1 antibody
      • M-phase inducer phosphatase 1 antibody
      • MGC115549 antibody
      • MPIP1_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Cdc25A antibody (ab989)
      Western blot - Anti-Cdc25A antibody (ab989)

      ab989 (Rabbit polyclonal to exon 7 of human Cdc25a) and ab991 (Rabbit polyclonal to exon 9 of human Cdc25a) both at a dilution of 1:2000, staining 500µg HeLa whole cell extract by Immunoprecipitation / Western Blot (ECL)

    参考文献 (9)

    ab989 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab989 は 9 報の論文で使用されています。

    • Das S  et al. Genome-scale screening of deubiquitinase subfamily identifies USP3 as a stabilizer of Cdc25A regulating cell cycle in cancer. Cell Death Differ N/A:N/A (2020). PubMed: 32415280
    • Wang Y  et al. P53 suppresses SENP3 phosphorylation to mediate G2 checkpoint. Cell Discov 6:21 (2020). PubMed: 32351703
    • Su J  et al. Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation. Mol Cell Probes 46:101415 (2019). PubMed: 31228519
    • Tu X  et al. ATR Inhibition Is a Promising Radiosensitizing Strategy for Triple-Negative Breast Cancer. Mol Cancer Ther 17:2462-2472 (2018). PubMed: 30166399
    • Martinez-Font E  et al. Disruption of TCF/ß-Catenin Binding Impairs Wnt Signaling and Induces Apoptosis in Soft Tissue Sarcoma Cells. Mol Cancer Ther 16:1166-1176 (2017). PubMed: 28292937
    • Zhang Z  et al. Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer. BMC Cancer 17:861 (2017). PubMed: 29246203
    • Liang J  et al. PKM2 dephosphorylation by Cdc25A promotes the Warburg effect and tumorigenesis. Nat Commun 7:12431 (2016). WB ; Human . PubMed: 27485204
    • Brunetto E  et al. CDC25A protein stability represents a previously unrecognized target of HER2 signaling in human breast cancer: implication for a potential clinical relevance in trastuzumab treatment. Neoplasia 15:579-90 (2013). PubMed: 23730206
    • Liu H  et al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB ; Human . PubMed: 20818375

    レビューと Q&A

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    Western blot abreview for Anti-Cdc25A antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT29 tumour cells)
    Loading amount
    50 µg
    Specification
    HT29 tumour cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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    Mr. Paul Eve

    Verified customer

    投稿 Jan 09 2007

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