Anti-CDA 抗体 (ab231981)
Key features and details
- Rabbit polyclonal to CDA
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-CDA antibody
CDA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CDA -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human, Pig -
免疫原
Recombinant full length protein (His-T7-tag) corresponding to Human CDA aa 1 to the C-terminus. Expresssed in E.coli.
Database link: P32320 -
ポジティブ・コントロール
- WB: Recombinant human CDA protein; HeLa whole cell lysate; Pig kidney tissue lysate. IHC-P: Human kidney, lung cancer, skin cancer and bile duct cancer tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab231981の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 16 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 16 kDa. |
ターゲット情報
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関連性
CDA (Cytidine deaminase) scavengers exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. Growth inhibition of granulocyte-macrophage colony foring cells by human cytidine deaminase requires the catalytic function of the protein. It is highly expressed in granulocytes. -
参照データベース
- Entrez Gene: 978 Human
- Omim: 123920 Human
- SwissProt: P32320 Human
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別名
- CDD antibody
- Cytidine aminohydrolase antibody
- Cytidine deaminase antibody
- EC 3.5.4.5 antibody
画像
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Formalin-fixed, paraffin-embedded human kidney tissue stained for CDA using ab231981 at 10 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human skin cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CDA using ab231981 at 20μg/ml in ICC/IF.
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Formalin-fixed, paraffin-embedded human bile duct cancer tissue stained for CDA using ab231981 at 20 μg/ml in immunohistochemical analysis.
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Anti-CDA antibody (ab231981) at 3 µg/ml + Recombinant human CDA
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa -
All lanes : Anti-CDA antibody (ab231981) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Pig kidney lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 16 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab231981 は論文での使用が確認できていません。