Anti-CD99 抗体 [DN16] (ab23617)
Key features and details
- Mouse monoclonal [DN16] to CD99
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CD99 antibody [DN16]
CD99 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [DN16] to CD99 -
由来種
Mouse -
特異性
ab23617 recognises the human CD99 cell surface antigen, a 32kD glycoprotein expressed by all leucocytes. CD99 expression tends to be highest on immature cells. -
アプリケーション
適用あり: IHC-Fr, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
The details of the immunogen for this antibody are not available.
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エピトープ
Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence 'LPDNENKK' located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gil et al. 2002). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
DN16 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23617の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. -
配列類似性
Belongs to the CD99 family. -
翻訳後修飾
Extensively O-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4267 Human
- Omim: 313470 Human
- SwissProt: P14209 Human
- Unigene: 653349 Human
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別名
- 12E7 antibody
- Antigen identified by monoclonal 12E7, Y homolog antibody
- Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab23617 は 4 報の論文で使用されています。
- Cardoso LC et al. CD99 Expression in Glioblastoma Molecular Subtypes and Role in Migration and Invasion. Int J Mol Sci 20:N/A (2019). PubMed: 30845661
- O'Neill AF et al. Targeted imaging of Ewing sarcoma in preclinical models using a 64Cu-labeled anti-CD99 antibody. Clin Cancer Res 20:678-87 (2014). PubMed: 24218512
- Rabquer BJ et al. The proadhesive phenotype of systemic sclerosis skin promotes myeloid cell adhesion via ICAM-1 and VCAM-1. Rheumatology (Oxford) 48:734-40 (2009). ELISA, IHC-Fr ; Human . PubMed: 19439502
- Kim SH et al. Viral latent membrane protein 1 (LMP-1)-induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype. Blood 95:294-300 (2000). PubMed: 10607715
