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    cd98-antibody-mem-108-plasma-membrane-marker-ab2528.pdf

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Immunology Cell Type Markers CD Non-lineage
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Anti-CD98 抗体 [MEM-108] - Plasma Membrane Marker (ab2528)

  • Datasheet
  • SDS
Submit a review Q&A (5)References (1)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)
  • Western blot - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)

Key features and details

  • Mouse monoclonal [MEM-108] to CD98 - Plasma Membrane Marker
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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関連製品

製品の概要

  • 製品名

    Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker
    CD98 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [MEM-108] to CD98 - Plasma Membrane Marker
  • 由来種

    Mouse
  • 特異性

    This antibody recognises CD98 antigen.
  • アプリケーション

    適用あり: WB, ICC/IFmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Tissue/ cell preparation (Human). Burkitt’s lymphoma cell line Raji.

  • ポジティブ・コントロール

    • ICC/IF: MCF7 cells.
  • 特記事項

    CD98 (4F2) is involved in normal and neoplastic cell growth.

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 特記事項(精製)

    This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE.
  • 一次抗体 備考

    CD98 (4F2) is involved in normal and neoplastic cell growth.
  • ポリ/モノ

    モノクローナル
  • クローン名

    MEM-108
  • アイソタイプ

    IgG1
  • 研究分野

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Alternative Versions

    • Biotin Anti-CD98 antibody [MEM-108] (ab26009)
    • FITC Anti-CD98 antibody [MEM-108] (ab26010)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD98 protein (ab181902)

アプリケーション

Our Abpromise guarantee covers the use of ab2528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier.
  • 組織特異性

    Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1.
  • 配列類似性

    Belongs to the SLC3A transporter family.
  • 翻訳後修飾

    Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions.
  • 細胞内局在

    Apical cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localized to the plasma membrane when associated with SLC7A5 or SLC7A8. Localized to the placental apical membrane. Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity).
  • Target information above from: UniProt accession P08195 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 6520 Human
    • Omim: 158070 Human
    • SwissProt: P08195 Human
    • Unigene: 502769 Human
    • 製品の状態

      There are 4 isoforms produced by alternative splicing.
    • 別名

      • 4F2 antibody
      • 4F2 cell surface antigen heavy chain antibody
      • 4F2 cell-surface antigen heavy chain antibody
      • 4F2 heavy chain antibody
      • 4F2 heavy chain antigen antibody
      • 4F2_HUMAN antibody
      • 4F2hc antibody
      • 4T2HC antibody
      • Antigen defined by monoclonal antibody 4F2 heavy chain antibody
      • Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43 antibody
      • CD 98 antibody
      • CD98 antibody
      • CD98 antigen antibody
      • CD98 heavy chain antibody
      • CD98HC antibody
      • Heavy chain antibody
      • Lymphocyte activation antigen 4F2 large subunit antibody
      • MDU 1 antibody
      • MDU1 antibody
      • Monoclonal antibody 44D7 antibody
      • NACAE antibody
      • Slc3a2 antibody
      • Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 antibody
      • Solute carrier family 3 member 2 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)
      Immunocytochemistry/ Immunofluorescence - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)

      ICC/IF image of ab2528 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2528, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)
      Western blot - Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528)
      All lanes : Anti-CD98 antibody [MEM-108] - Plasma Membrane Marker (ab2528) at 5 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution


      The CD98 protein has a predicted molecular weight of 57 kDa, however it is extensively glycosylated. This antibody recognises a bands at 98-150 kDa, similar to what has been observed with other antibodies directed against this protein.  

      Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    プロトコール

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (1)

    ab2528 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2528 は 1 報の論文で使用されています。

    • Millen S  et al. Collagen IV (COL4A1, COL4A2), a Component of the Viral Biofilm, Is Induced by the HTLV-1 Oncoprotein Tax and Impacts Virus Transmission. Front Microbiol 10:2439 (2019). PubMed: 31708905

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Question

    Thank you for your email. We've now rerun the samples and unfortunately it's still not very good.
    We know the cells are definitely positive for CD98 because we've done extracellular flow cytometry on them and they stain nicely (have very high levels). Our lysis protocol works nicely because we see good staining of mouse tumour cells with a different CD98 antibody.

    Please find attached a picture of 2 westerns done using your suggested modifications.
    The first was detecting using a pico-HRP substrate (all we are detecting is marker) and the second is using a much more sensitive femto reagent. We know the proteins transferred well to the membrane, there is just very little sensitivity and a lot of background (the third lane in each occasion is molecular weight markers. The femto reagent had to be exposed for a long time as you can see from the marker lane.

    Would it be possible to get a refund on this product please as we've now bought a replacement antibody from a different company?
    Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Thank you for your email.
    I am sorry to hear that the product failed again. I have now raised the quality issues to our qc team. They might decide to retest this product.
    I appreciate the time you have spent in troubleshooting and for the cooperation while dealing with this case. It is very unfortunate that the product did not give expected results.
    I have put forward a request to our accounting department for refund. The refund ID is 23079. The refund note may be used in one of the following ways:
    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2) Held on the account for use against a future order.
    (3) A full refund can be offered where no other invoices are outstanding.
    Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
    To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
    The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

    Read More

    Abcam Scientific Support

    Answered on Oct 17 2012

    Question

    Please find attached the questionnaire regarding our recent antibody experiences. I've also attached a jpg of the CD98 and control western blot stains.
    It would be good to get an alternative antibody as soon as possible as we need to get this project moving forward.
    Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 09 2012

    Answer

    Thank you for contacting us. I apologies for the delay.
    The protocol seems fine to me however I would suggest following updates;
    - Blocking time; I can recommend blocking the membrane for 1-2 hours in 5% BSA at RT, I recommended BSA because it works better with some antibodies which is why we commonly use it in our testing lab.
    - Heat the protein in sample buffer for 10 minutes at 100C.
    Please note as CD98 is a heavily glycosylated protein so the appearance of band will be like smear rather than sharp one.
    I am sure these simple changes will help to improve results. Please do not hesitate to contact me if antibody fails again.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 09 2012

    Question

    - Antibody fails in WB
    - Multiple bands around band of interest

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2012

    Answer

    Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    As we discussed on the phone, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2012

    Question

    Inquiry: The CD98 protein is a heterodimer, composed of a heavy chain and a light chain linked by disulfide bonds. We're looking for an antibody staining ONLY the HEAVY CHAIN of CD98 (also named CD98hc, 4F2hc or SLC3A2) by immunofluorescence. The antibody clone MEM-108 is described as recognizing CD98, but it is not clear whether it would stains one or the other (or both) of the chains. The western-blot shown in the datasheet does not give a clear answer because the high molecular weigth signal suggests that the western-blot was done on a native gel (the expected size of the heterodimer is 125kDa whereas the heavy chain is approx. 80kDa and the light-chain 40kDa). Even if the heavy chain is highly glycosylated, we should expect a smaller band than what is shown on the figure... Do you have more info about the specificity of this antibody or about how this western-blot was performed ? Many thanks in advance for your help.

    Read More

    Abcam community

    Verified customer

    Asked on May 31 2012

    Answer

    Thank you for contacting us and sorry for the delay of our reply.

    Please find below the information I obtained for each anti-CD98 antibodies:

    ab2528 : the epitope hasnot beenmapped.
    ab23495 [44D7] reacts with the heavy chain of the CD98 molecule. Tested in ICC/IF.
    ab108300 [EPR3548(2)] recognises the extracellular region of the human CD98 (heavy chain).



    I would recommend the antibody https://www.abcam.com/ab108300.
    To our knowledge,ab108300 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab108300 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab108300 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab108300 so please wait for my reply before ordering.

    2. Purchase ab108300 either by phone, fax, or online (https://www.abcam.com).

    3. Test it in ICC/IF.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab108300 turns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on May 31 2012

    Question

    Dear Sir/Madame, do you have any info on the reactivity of anti CD98 antibody with rat cells?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 21 2004

    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

    Read More

    Abcam Scientific Support

    Answered on Apr 21 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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