Anti-CD98 抗体 [MEM-108] - Plasma Membrane Marker (ab2528)

Thank you for your email.
I am sorry to hear that the product failed again. I have now raised the quality issues to our qc team. They might decide to retest this product.
I appreciate the time you have spent in troubleshooting and for the cooperation while dealing with this case. It is very unfortunate that the product did not give expected results.
I have put forward a request to our accounting department for refund. The refund ID is 23079. The refund note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for contacting us. I apologies for the delay.
The protocol seems fine to me however I would suggest following updates;
- Blocking time; I can recommend blocking the membrane for 1-2 hours in 5% BSA at RT, I recommended BSA because it works better with some antibodies which is why we commonly use it in our testing lab.
- Heat the protein in sample buffer for 10 minutes at 100C.
Please note as CD98 is a heavily glycosylated protein so the appearance of band will be like smear rather than sharp one.
I am sure these simple changes will help to improve results. Please do not hesitate to contact me if antibody fails again.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
As we discussed on the phone, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.

Thank you for contacting us and sorry for the delay of our reply.

Please find below the information I obtained for each anti-CD98 antibodies:

ab2528 : the epitope hasnot beenmapped.
ab23495 [44D7] reacts with the heavy chain of the CD98 molecule. Tested in ICC/IF.
ab108300 [EPR3548(2)] recognises the extracellular region of the human CD98 (heavy chain).



I would recommend the antibody https://www.abcam.com/ab108300.
To our knowledge,ab108300 has not been tested in ICC/IF. Therefore, I can offer a discount off a future purchase if you buy ab108300 now, test it in ICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab108300 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab108300 so please wait for my reply before ordering.

2. Purchase ab108300 either by phone, fax, or online (https://www.abcam.com).

3. Test it in ICC/IF.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab108300 turns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.