Anti-CD94 抗体 [EPR23229-138] - BSA and Azide free (ab270752)
![Flow Cytometry - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)](https://www.abcam.co.jp/ps/products/270/ab270752/Images/ab270752-383493-anticd94antibodyepr23229138flowcytometryjurkat.jpg "Flow Cytometry - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23229-138] to CD94 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
製品の概要
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製品名
Anti-CD94 antibody [EPR23229-138] - BSA and Azide free
CD94 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23229-138] to CD94 - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: NK-92 cell. Flow Cyt: Jurkat cell.
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特記事項
ab270752 is the carrier-free version of ab245240. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23229-138 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
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| ICC/IF |
Human
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| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. -
組織特異性
Natural killer cells. -
配列類似性
Contains 1 C-type lectin domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3824 Human
- Omim: 602894 Human
- SwissProt: Q13241 Human
- Unigene: 562457 Human
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別名
- CD 94 antibody
- CD94 antibody
- CD94 antigen antibody
see all
画像
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Flow Cytometry - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell, Right) cells labelling CD94 with ab245240 at 1/500 dilution (0.1ug)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID: 9034158)
Gated on viable cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245240).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)](https://www.abcam.co.jp/ps/products/270/ab270752/Images/ab270752-1-anti-cd94-antibody-epr23229138-immunocytochemistry-nk92.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 cells labelling CD94 with ab245240 at 1/100 5 ug/ml dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in NK-92 cell line is observed.
Negative control: Jurkat PMID: 9034158.Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245240).
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![Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)](https://www.abcam.co.jp/ps/products/270/ab270752/Images/ab270752-19-benefits-of-recombinant-antibodies.png)
Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab270752 は論文での使用が確認できていません。