製品の概要

  • 製品名
    Anti-CD90 / Thy1 antibody [MRC OX-7] (FITC)
    CD90 / Thy1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MRC OX-7] to CD90 / Thy1 (FITC)
  • 由来種
    Mouse
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c.
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Guinea pig
  • 免疫原

    Full length protein (Rat).

  • 特記事項

    The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

    Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能
    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • 配列類似性
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD7 antibody
    • CD90 antibody
    • CD90 antigen antibody
    • CDw90 antibody
    • FLJ33325 antibody
    • MGC128895 antibody
    • T25 antibody
    • Theta antigen antibody
    • Thy 1 antibody
    • Thy 1 cell surface antigen antibody
    • Thy 1 membrane glycoprotein antibody
    • Thy 1 T cell antigen antibody
    • Thy 1.2 antibody
    • Thy-1 antigen antibody
    • Thy-1 membrane glycoprotein antibody
    • Thy1 antibody
    • Thy1 antigen antibody
    • Thy1 T cell antigen antibody
    • Thy1.1 antibody
    • Thy1.2 antibody
    • THY1_HUMAN antibody
    • Thymus cell antigen 1, theta antibody
    see all

画像

  • ab226 staining CD90 / Thy1 (FITC) in rat thymus cells by Flow Cytometry analysis.

プロトコール

参考文献

This product has been referenced in:
  • Sun AJ  et al. Comparison of mouse brown and white adipose-derived stem cell differentiation into pacemaker-like cells induced by TBX18 transduction. Mol Med Rep 17:7055-7064 (2018). Read more (PubMed: 29568953) »
  • Tarafder S  et al. Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment. Sci Rep 8:8150 (2018). Read more (PubMed: 29802356) »
See all 14 Publications for this product

レビューと Q&A

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us and reporting the problems you have experienced in using ab226. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab226. I would also appreciate if you can confirm some further details: 1. What washing steps were preformed? And with what buffers? 2. What volume of buffer was the cells suspended in for the primary antibody incubation? 3. Was the antibody kept in the dark at all times? 4. Was any analysis performed to detect the dead cells within the sample? I would suggest trying to use a higher concentration of primary antibody. The directions supplied with the datasheet are only guidelines and may need to be optimised for your particular experiment. I would suggest using a 1/10 dilution. It may also be worth trying a different blocking agent such as 3% BSA in PBS. Care should also be taken to keep the sample ice cold when preparing and I would suggest incubating the antibody with the cells (in the dark) for 1 hour at 4°C. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

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Answer

Thank you for contacting us. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. I found conflicting information about the expression of CD90 on NIH3T3 cells: No expression or expression of Thy1.2 (CD90.2). ab226 only recognizes Thy1.1 (CD90.1). I therefore suggest to use ab25672 (Anti-CD90 / Thy1 antibody [G7] (FITC)) which will bind CD90.1 and CD90.2. I could not find any reliable data on the expression of Ly6 on NIH3T3 cells either, but can confirm that ab25031 will bind to Ly6A.2 and Ly6E. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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