Anti-CD90 / Thy1 抗体 [EPR3133] - Low endotoxin, Azide free (ab216449)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3133] to CD90 / Thy1 - Low endotoxin, Azide free
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
製品の概要
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製品名
Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free
CD90 / Thy1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3133] to CD90 / Thy1 - Low endotoxin, Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC: Human tonsil tissue; WB: Human glioma and brain tissue lysate; ICC/IF: Jurkat cells.
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特記事項
ab216449 is the carrier-free version of ab133350.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3133 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-CD90 / Thy1 antibody [EPR3133] (ab133350)
- Alexa Fluor® 568 Anti-CD90 / Thy1 antibody [EPR3133] (ab201848)
- Alexa Fluor® 647 Anti-CD90 / Thy1 antibody [EPR3133] (ab202334)
- Alexa Fluor® 555 Anti-CD90 / Thy1 antibody [EPR3133] (ab202511)
- Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR3133] (ab202512)
- Anti-CD90 / Thy1 antibody [EPR3133] - BSA and Azide free (ab226123)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab216449の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
Observed molecular weight may vary depending on the glycosylation level of the target. |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa. Observed molecular weight may vary depending on the glycosylation level of the target. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7070 Human
- Omim: 188230 Human
- SwissProt: P04216 Human
- Unigene: 644697 Human
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別名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
画像
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All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/2000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lane 3 : Human brain cell lysate
Lane 4 : Human kidney cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 25-37 kDa why is the actual band size different from the predicted?This data was developed using ab133350, the same antibody clone in a different buffer formulation.
Anti-THY1 antibody [EPR3133] (ab133350) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133350 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-CD90 / Thy1 antibody [EPR3133] (ab133350) at 1/1000 dilution (Purified)
Lane 1 : Human glioma lysate
Lane 2 : Human brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 25-35 kDa why is the actual band size different from the predicted?This data was developed using ab133350, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID: 24116172 and PMID: 30177788).
Blocking Buffer and concentration: 5% NFDM/TBST
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This data was developed using ab133350, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD90 / Thy1 with Purified ab216449 at 1:50 dilution (2.44 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
This data was developed using ab133350, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD90 / Thy1 with Purified ab216449 at 1:4000 dilution (0.03 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
プロトコール
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (4)
ab216449 は 4 報の論文で使用されています。
- Liu Y et al. Mongolian Medicine echinops prevented postmenopausal osteoporosis and induced ER/AKT/ERK pathway in BMSCs. Biosci Trends 12:275-281 (2018). PubMed: 29794404
- Kawasaki T et al. Six1 is required for mouse dental follicle cell and human periodontal ligament-derived cell proliferation. Dev Growth Differ N/A:N/A (2016). IF . PubMed: 27241908
- Skowron MA et al. Phenotype plasticity rather than repopulation from CD90/CK14+ cancer stem cells leads to cisplatin resistance of urothelial carcinoma cell lines. J Exp Clin Cancer Res 34:144 (2015). ICC/IF ; Human . PubMed: 26606927
- Ramírez G et al. Absence of Thy-1 results in TGF-ß induced MMP-9 expression and confers a profibrotic phenotype to human lung fibroblasts. Lab Invest 91:1206-18 (2011). WB, IHC-P ; Human . PubMed: 21577212