Anti-CD89 抗体 [EPR4622(2)] - BSA and Azide free (ab247978)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4622(2)] to CD89 - BSA and Azide free
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD89 antibody [EPR4622(2)] - BSA and Azide free
CD89 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4622(2)] to CD89 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details
適用なし: Flow Cyt (Intra) -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: U937, K562, Jurkat, and HL-60 cell lysates; IHC: Human tonsil tissue; IP: U-937.
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特記事項
ab247978 is the carrier-free version of ab124717.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4622(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247978の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production. -
組織特異性
Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2204 Human
- Omim: 147045 Human
- SwissProt: P24071 Human
- Unigene: 659872 Human
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別名
- CD89 antibody
- Fc alpha receptor antibody
- Fc fragment of IgA, receptor for antibody
see all
画像
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All lanes : Anti-CD89 antibody [EPR4622(2)] (ab124717) at 1/1000 dilution
Lane 1 : U937 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : HL-60 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDaThis data was developed using ab124717, the same antibody clone in a different buffer formulation.
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This data was developed using ab124717, the same antibody clone in a different buffer formulation.
CD89 was immunoprecipitated from 0.35 mg U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10 µg with ab124717 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10 µg
Lane 2: abab124717 IP in U-937 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124717 in U-937 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247978 は論文での使用が確認できていません。