Anti-CD86 抗体 [EPR21962] (ab239075)

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Flow Cytometry - Anti-CD86 antibody [EPR21962] (ab239075)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21962] to CD86
Suitable for: WB, ICC/IF, Flow Cyt, IP
Knockout validated
Reacts with: Human

製品名

Anti-CD86 antibody [EPR21962]
CD86 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR21962] to CD86
由来種

Rabbit
アプリケーション

適用あり: WB, ICC/IF, Flow Cyt, IPmore details
適用なし: IHC-P
種交差性

交差種: Human
免疫原

Recombinant fragment within Human CD86 aa 1-250. The exact sequence is proprietary.
Database link: P42081
ポジティブ･コントロール
- WB: Ramos, Raji and Daudi whole cell lysates. ICC/IF: Raji and Daudi cells. Flow Cyt: Raji cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR21962
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-CD86 antibody [EPR21962] - BSA and Azide free (ab242020)
Compatible Secondaries
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
ELISA pair antibody
- Recombinant human CD86 protein (Fc Chimera) (ab167720)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human CD86 protein (Fc Chimera) (ab167720)
Related Buffer
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)

Our Abpromise guarantee covers the use of ab239075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
WB		1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 38 kDa).
ICC/IF		1/100.
Flow Cyt		1/500.
IP		1/30.

追加情報

Is unsuitable for IHC-P.

機能

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
組織特異性

Expressed by activated B-lymphocytes and monocytes.
配列類似性

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
翻訳後修飾

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
細胞内局在

Membrane.
Target information above from: UniProt accession P42081 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
別名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
- B-lymphocyte activation antigen B7-2 antibody
- B7 2 antibody
- B7 antibody
- B7-2 antibody
- B7.2 antibody
- B70 antibody
- B72 antibody
- B72 antigen antibody
- BU63 antibody
- CD28 antigen ligand 2 2 antibody
- CD28 antigen ligand 2 antibody
- Cd28l2 antibody
- CD28LG2 antibody
- CD86 antibody
- CD86 antigen (CD28 antigen ligand 2 B7 2 antigen) antibody
- CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
- CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
- CD86 antigen antibody
- CD86 molecule antibody
- CD86_HUMAN antibody
- CLS1 antibody
- CTLA-4 counter-receptor B7.2 2 antibody
- CTLA-4 counter-receptor B7.2 2, 3 antibody
- CTLA-4 counter-receptor B7.2 antibody
- Early T-cell costimulatory molecule 1 antibody
- ETC-1 antibody
- FUN 1 antibody
- FUN-1 antibody
- FUN1 antibody
- LAB72 antibody
- Ly-58 antibody
- Ly58 antibody
- MB7 antibody
- MB7-2 antibody
- Membrane glycoprotein antibody
- MGC34413 antibody
- T lymphocyte activation antigen CD86 precursor antibody
- T-lymphocyte activation antigen CD86 antibody
- TS/A-2 antibody
see all

Flow Cytometry - Anti-CD86 antibody [EPR21962] (ab239075)

Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD86 with ab239075 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.
Immunocytochemistry/ Immunofluorescence - Anti-CD86 antibody [EPR21962] (ab239075)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi (human Burkitt's lymphoma cell line) cells labeling CD86 with ab239075 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Daudi cell line is observed.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-CD86 antibody [EPR21962] (ab239075)

All lanes : Anti-CD86 antibody [EPR21962] (ab239075) at 1/1000 dilution

Lane 1 : Wild-type Raji cell lysate
Lane 2 : CD86 knockout Raji cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 38 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Lanes 1 - 2: Merged signal (red and green). Green - ab239075 observed at 70 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab239075 was shown to react with CD86 in wild-type Raji cells in western blot with loss of signal observed in CD86 knockout sample. Wild-type and CD86 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab239075 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-CD86 antibody [EPR21962] (ab239075)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma cell line) cells labeling CD86 with ab239075 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Raji cell line is observed.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-CD86 antibody [EPR21962] (ab239075)

CD86 was immunoprecipitated from 0.35 mg of Raji whole (human Burkitt's lymphoma cell line) cell lysate with ab239075 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab239075 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1: Raji whole cell lysate 10 µg (Input).

Lane 2: ab239075 IP in Raji whole lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239075 in Raji whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-CD86 antibody [EPR21962] (ab239075)

All lanes : Anti-CD86 antibody [EPR21962] (ab239075) at 1/1000 dilution

Lane 1 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Daudi (human Burkitt's lymphoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 38 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?

Exposure time : Lane 1: 3 minutes; Lanes 2-3: 26 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CD86 antibody [EPR21962] (ab239075)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet

ab239075 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab239075 は 1 報の論文で使用されています。

Zhang C et al. miR-30c-5p Reduces Renal Ischemia-Reperfusion Involving Macrophage. Med Sci Monit 25:4362-4369 (2019). PubMed: 31185006

レビューと Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Conjugation kits

ELISA pair antibody

Isotype control

Recombinant Protein

Related Buffer

アプリケーション

ターゲット情報

機能

組織特異性

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Certificate of Compliance

参考文献 (1)

レビューと Q&A