Anti-CD86 抗体 [EP1158-37] (ab269587)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EP1158-37] (ab269587)](https://www.abcam.co.jp/ps/products/269/ab269587/Images/ab269587-2-anti-cd86-antibody-ep1158-37-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EP1158-37] (ab269587)")
Key features and details
- Rabbit monoclonal [EP1158-37] to CD86
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CD86 antibody [EP1158-37]
CD86 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1158-37] to CD86 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Raji, Daudi, Jurkat, Human tonsil and Human spleen lysates. IHC-P: Human non-Hodgkin lymphoma and Human tonsil tissues.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1158-37 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC |
Human
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| IHC-P |
Human
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| IP |
Human
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| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 38 kDa).
|
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 38 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
組織特異性
Expressed by activated B-lymphocytes and monocytes. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
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別名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EP1158-37] (ab269587)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD86 with ab269587 at 1/100 dilution (2.52 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 22740445). The section was incubated with ab269587 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Western blot - Anti-CD86 antibody [EP1158-37] (ab269587)](https://www.abcam.co.jp/ps/products/269/ab269587/Images/ab269587-448037-anti-cd86-antibody-ep1158-37-western-blot-raji-wt-raji-cd86-knockout.jpg)
Western blot - Anti-CD86 antibody [EP1158-37] (ab269587)All lanes : Anti-CD86 antibody [EP1158-37] (ab269587) at 1/1000 dilution
Lane 1 : Wild-type Raji cell lysate
Lane 2 : CD86 knockout Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab269587 observed at 70 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab269587 was shown to react with CD86 in wild-type Raji cells in western blot with loss of signal observed in CD86 knockout sample. Wild-type and CD86 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab269587 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CD86 antibody [EP1158-37] (ab269587)](https://www.abcam.co.jp/ps/products/269/ab269587/Images/ab269587-4-anti-cd86-antibody-ep1158-37-western-blot-human.jpg)
Western blot - Anti-CD86 antibody [EP1158-37] (ab269587)All lanes : Anti-CD86 antibody [EP1158-37] (ab269587) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Daudi (human burkitt's lymphoma lymphoblast) whole cell lysate
Lane 3 : Jurkat (human t cell leukemia t lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBSt.
Exposure time: 3 minutes.
Negative control: Jurkat (PMID: 9973476).
The actual band size higher than predicted is mainly due to glycosylation.
The molecular weight observed is consistent with what has been described in the literature (PMID:27464357, 21896490).
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![Western blot - Anti-CD86 antibody [EP1158-37] (ab269587)](https://www.abcam.co.jp/ps/products/269/ab269587/Images/ab269587-3-anti-cd86-antibody-ep1158-37-western-blot-human-2.jpg)
Western blot - Anti-CD86 antibody [EP1158-37] (ab269587)All lanes : Anti-CD86 antibody [EP1158-37] (ab269587) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
The actual band size higher than predicted is mainly due to glycosylation.
The molecular weight observed is consistent with what has been described in the literature (PMID:27464357, 21896490).
This blot was developed using a higher sensitivity ECL substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EP1158-37] (ab269587)](https://www.abcam.co.jp/ps/products/269/ab269587/Images/ab269587-1-anti-cd86-antibody-ep1158-37-immunohistochemistry-nonHodgkin-lymphoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD86 antibody [EP1158-37] (ab269587)Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin lymphoma tissue labeling CD86 with ab269587 at 1/100 dilution (2.52 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human non-Hodgkin lymphoma (PMID: 9767448,10089910). The section was incubated with ab269587 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献 (0)
ab269587 は論文での使用が確認できていません。
