Anti-CD80 抗体 [EPR1157(2)] (ab134120)
![Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.co.jp/ps/products/134/ab134120/Images/ab134120-333814-anti-cd80-antibody-epr11572-flow-cytometry.jpg "Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1157(2)] to CD80
- Suitable for: Flow Cyt, WB, IHC-P
- Knockout validated
- Reacts with: Human
製品の概要
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製品名
Anti-CD80 antibody [EPR1157(2)]
CD80 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1157(2)] to CD80 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, WB, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD80 aa 250 to the C-terminus.
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ポジティブ･コントロール
- WB: Raji and Ramos cell lysates. IHC-P: human tonsil Tissue Flow Cyt: Raji cells
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特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1157(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab134120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa). For unpurified use at 1/500. |
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| IHC-P | 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
組織特異性
Expressed on activated B-cells, macrophages and dendritic cells. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 941 Human
- Omim: 112203 Human
- SwissProt: P33681 Human
- Unigene: 838 Human
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別名
- Activation B7-1 antigen antibody
- B lymphocyte activation antigen B7 antibody
- B7 antibody
see all
画像
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Flow Cytometry - Anti-CD80 antibody [EPR1157(2)] (ab134120)Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD80 with purified ab134120 at 1:190 dilution (10.33 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.co.jp/ps/products/134/ab134120/Images/ab134120-269698-anti-cd80-antibody-epr11572-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD80 antibody [EPR1157(2)] (ab134120)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD80 with ab134120 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Trs/EDTA buffer, pH9 (ab93684). ImmunoHistoProbe one step HRP Polymer was used. Counter stained with hematoxylin.
The image shows cytoplasmic and membrane staining.
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![Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.co.jp/ps/products/134/ab134120/Images/ab134120-415035-anti-cd80-antibody-epr11572-western-blot-raji-wt-raji-cd80-knockout-ramos.jpg)
Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution
Lane 1 : Wild-type Raji cell lysate
Lane 2 : CD80 knockout Raji cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab134120 observed at 55 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab134120 was shown to react with CD80 in wild-type Raji cells in western blot with loss of signal observed in CD80 knockout sample. Wild-type and CD80 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134120 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.co.jp/ps/products/134/ab134120/Images/ab134120-239863-anti-cd80-antibody-epr11572-western-blot.jpg)
Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/2500 dilution (purified) + Raji cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.co.jp/ps/products/134/ab134120/Images/ab134120-148331-anti-cd80-antibody-epr11572-western-blot.jpg)
Western blot - Anti-CD80 antibody [EPR1157(2)] (ab134120)All lanes : Anti-CD80 antibody [EPR1157(2)] (ab134120) at 1/1000 dilution (unpurified)
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Anti-CD80 antibody [EPR1157(2)] (ab134120)](https://www.abcam.co.jp/ps/products/134/ab134120/Images/ab134120-8-benefits-of-recombinant-antibodies.png)
Anti-CD80 antibody [EPR1157(2)] (ab134120)
プロトコール
参考文献 (11)
ab134120 は 11 報の論文で使用されています。
- Amponsa VO et al. Carcinogen-induced bladder cancer in the FVB mouse strain is associated with glandular differentiation and increased Cd274/Pdl-1 expression. Am J Clin Exp Urol 7:139-152 (2019). PubMed: 31317053
- Kuwabara S et al. Prognostic relevance of tertiary lymphoid organs following neoadjuvant chemoradiotherapy in pancreatic ductal adenocarcinoma. Cancer Sci 110:1853-1862 (2019). PubMed: 30997706
- Cui CY et al. Skewed macrophage polarization in aging skeletal muscle. Aging Cell 18:e13032 (2019). PubMed: 31478346
- Nascimento Da Conceicao V et al. Loss of Ca2+ entry via Orai-TRPC1 induces ER stress, initiating immune activation in macrophages. J Cell Sci 133:N/A (2019). PubMed: 31722977
- Gunasekaran M et al. Circulating Exosomes with Distinct Properties during Chronic Lung Allograft Rejection. J Immunol 200:2535-2541 (2018). PubMed: 29491008
