Anti-CD8 alpha 抗体 [SP239] (ab178089)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP239] to CD8 alpha
- Suitable for: mIHC, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD8 alpha antibody [SP239]
CD8 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP239] to CD8 alpha -
由来種
Rabbit -
アプリケーション
適用あり: mIHC, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P and mIHC: Human tonsil tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SP239 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab178089の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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mIHC |
1/100.
|
|
IHC-P | (1) |
1/100.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
特記事項 |
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mIHC
1/100. |
IHC-P
1/100. Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
ターゲット情報
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機能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
関連疾患
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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別名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling PD1 with ab243644 at 1/500 dilution (1.02 µg/mL) (D), CD8 with ab178089 at 1/100 dilution (0.83 μg/ml) (C) and TIM 3 with ab242080 at 1/500 dilution (2.09 μg/ml) (B). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Panel A: merged staining of anti-TIM 3 (green; Opal™690), anti-CD8 (red; Opal™520) and anti-PD1 (gray; Opal™570) on human tonsil.
Panel B: anti-TIM 3 stained on membrane of a subset of immune cells.
Panel C: anti-CD8 stained on membrane of a subset of T cells.
Panel D: anti-PD1 stained on membrane of a subset of lymphocytes.
The section was incubated in three rounds of staining: in the order of ab242080, ab243644 and ab178089 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab178089 at followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil, performed on a Leica Biosystems BOND® RX instrument. Counterstained with Paraffin-embedded sections. Hematoxylin Secondary antibody only control: Used PBS instead of primary antibody.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD8 alpha with ab178089 at 1/100 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab178089 は 16 報の論文で使用されています。
- Zhang W et al. Serum Concentration of CD137 and Tumor Infiltration by M1 Macrophages Predict the Response to Sintilimab plus Bevacizumab Biosimilar in Advanced Hepatocellular Carcinoma Patients. Clin Cancer Res 28:3499-3508 (2022). PubMed: 35275208
- Wang J et al. Spatial Metabolomics Identifies Distinct Tumor-Specific Subtypes in Gastric Cancer Patients. Clin Cancer Res 28:2865-2877 (2022). PubMed: 35395077
- Sun L et al. Successful secondary radical operation on unretractable metastatic platinum-sensitive recurrent ovarian cancer by immunotherapy: a case report. Ann Transl Med 10:718 (2022). PubMed: 35845511
- Tong G et al. Intratumoral CD8+ T cells as a potential positive predictor of chemoimmunotherapy response in PD-L1-negative advanced gastric cancer patients: a retrospective cohort study. J Gastrointest Oncol 13:1668-1678 (2022). PubMed: 36092315
- Ju WT et al. A pilot study of neoadjuvant combination of anti-PD-1 camrelizumab and VEGFR2 inhibitor apatinib for locally advanced resectable oral squamous cell carcinoma. Nat Commun 13:5378 (2022). PubMed: 36104359