Anti-CD8 alpha 抗体 [EP1150Y] - BSA and Azide free (ab271868)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free (ab271868)](https://www.abcam.co.jp/ps/products/271/ab271868/Images/ab271868-2-ab93278253007anticd8antibodyep1150yimmunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free (ab271868)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1150Y] to CD8 alpha - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
製品の概要
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製品名
Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free
CD8 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1150Y] to CD8 alpha - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Human tonsil tissue.
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特記事項
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
ab271868 is the carrier-free version of ab93278. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1150Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
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| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
See IHC antigen retrieval protocols.This product showed specific staining in lymphocytes although we observed background staining in some epithelial cells tested.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. See IHC antigen retrieval protocols.This product showed specific staining in lymphocytes although we observed background staining in some epithelial cells tested.
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WB
Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
関連疾患
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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別名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free (ab271868)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD8 alpha with purified ab93278 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93278).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free (ab271868)](https://www.abcam.co.jp/ps/products/271/ab271868/Images/ab271868-1-ab93278108374anticd8antibodyep1150yimmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free (ab271868)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil labelling CD8 alpha with unpurified ab93278 at a dilution of 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93278).
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![Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free (ab271868)](https://www.abcam.co.jp/ps/products/271/ab271868/Images/ab271868-3-benefits-of-recombinant-antibodies.png)
Anti-CD8 alpha antibody [EP1150Y] - BSA and Azide free (ab271868)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab271868 は論文での使用が確認できていません。