Application
Flow Cytometry
Sample
Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization
Yes - 0.5% saponin
Gating Strategy
lymphocytes based on FSC vs SSC
Specification
peripheral blood mononuclear cells (PBMC)
Fixation
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: permeabilization buffer
Dilution
1/100
Secondary antibody
Dilution
1/800
Name
Non-Abcam antibody was used: F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Cells were fixed in 4% paraformaldehyde for 20 min on ice prior to permeabilization.
permeabilization buffer = PBS + 0.5% saponin, 2% FCS, 1mM EDTA, 0.1% sodium azide
After permeabilization, blocking was performed with 5% goat serum diluted in PBS for 30 min on ice.
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投稿 Oct 13 2016