Anti-CD8 alpha 抗体 [CAL38] (ab237723)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL38] to CD8 alpha
- Suitable for: IHC-Fr, IP, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD8 alpha antibody [CAL38]
CD8 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [CAL38] to CD8 alpha -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, IP, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Mouse spleen tissue. IHC-Fr: Mouse Thymus tissue. IP: Mouse thymus tissue lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purity >99%. -
ポリ/モノ
モノクローナル -
クローン名
CAL38 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab237723の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
1/250.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
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IP |
1/20.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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IHC-Fr
1/250. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
IP
1/20. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
関連疾患
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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別名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab237723 at 1/500 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on the mouse spleen is shown. The section was incubated with ab237723 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD8 alpha with ab237723 at 1/250 dilution, follwed by a AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution. Positive staining on the membrane of T cells in mouse thymus (PMID: 29042640) is observed. Counterstained with DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution.
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CD8 alpha was immunoprecipitated from 0.35 mg of Mouse thymus tissue lysate with ab237723 at 1/20 dilution. Western blot was performed on the immunoprecipitate using ab237723 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: Mouse thymus tissue lysate 10µg (input)
Lane 2: ab237723 IP in mouse thymus tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237723 in Mouse thymus lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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Formalin-fixed, paraffin-embedded mouse spleen tissue stained for CD8 alpha with ab237723 at 0.125 µg/ml in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab237723 は 5 報の論文で使用されています。
- Zheng Y et al. Radiation combined with KRAS-MEK inhibitors enhances anticancer immunity in KRAS-mutated tumor models. Transl Res 252:79-90 (2023). PubMed: 35948200
- Lu M et al. FGFR1 promotes tumor immune evasion via YAP-mediated PD-L1 expression upregulation in lung squamous cell carcinoma. Cell Immunol 379:104577 (2022). PubMed: 35870429
- Guo W et al. Ebosin Ameliorates Psoriasis-Like Inflammation of Mice via miR-155 Targeting tnfaip3 on IL-17 Pathway. Front Immunol 12:662362 (2021). PubMed: 33981308
- Rodriguez AB et al. Immune mechanisms orchestrate tertiary lymphoid structures in tumors via cancer-associated fibroblasts. Cell Rep 36:109422 (2021). PubMed: 34289373
- Leick KM et al. Patterns of immune-cell infiltration in murine models of melanoma: roles of antigen and tissue site in creating inflamed tumors. Cancer Immunol Immunother 68:1121-1132 (2019). PubMed: 31134297