Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0 Bond Epitope Retrieval Solution 2 (ER2, Leica)
Sample
Rhesus monkey Tissue sections (Spleen)
Specification
Spleen
Permeabilization
No
Fixative
10% Neutral Buffered Formalin
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Novocastra Bond Primary Antibody Diluent
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Secondary Goat anti-Rabbit and Tertiary Horse anti
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Signal:noise is acceptable and CD8-specific staining is comfortably demonstrated. Background is very low.
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投稿 Mar 26 2015