Anti-CD79a 抗体 [ZL7-4] (ab270256)

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Flow Cytometry - Anti-CD79a antibody [ZL7-4] (ab270256)
  • Immunofluorescence - Anti-CD79a antibody [ZL7-4] (ab270256)

Key features and details

  • Mouse monoclonal [ZL7-4] to CD79a
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD79a antibody [ZL7-4]
    CD79a 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [ZL7-4] to CD79a

  • 由来種

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IF
    Human
    See all applications and species data

  • 免疫原

    Tissue, cells or virus corresponding to Human CD79a. IgM complex isolated from Daudi cells.

  • ポジティブ･コントロール

    • Flow: Raji cells. ICC/IF: Raji cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
IF
Human
アプリケーション Abreviews 特記事項
IF
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.
特記事項
IF
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.

ターゲット情報

  • 機能

    Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.

  • 組織特異性

    B-cells.

  • 関連疾患

    Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.

  • 配列類似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.

  • 翻訳後修飾

    Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.

  • 細胞内局在

    Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Information by UniProt

  • 参照データベース

    • 別名

      • B lymphocyte-specific MB1 protein antibody
      • B-cell antigen receptor complex-associated protein alpha chain antibody
      • CD 79a antibody
      • CD79a antibody
      • CD79a antigen (immunoglobulin-associated alpha) antibody
      • CD79A antigen antibody
      • CD79a molecule, immunoglobulin-associated alpha antibody
      • CD79A_HUMAN antibody
      • Ig alpha antibody
      • Ig-alpha antibody
      • IGA antibody
      • IgM-alpha antibody
      • Immunoglobulin-associated alpha antibody
      • Ly54 antibody
      • MB-1 membrane glycoprotein antibody
      • MB1 antibody
      • Membrane-bound immunoglobulin-associated protein antibody
      • Surface IgM-associated protein antibody
      see all

    画像

    • Flow Cytometry - Anti-CD79a antibody [ZL7-4] (ab270256)
      Flow Cytometry - Anti-CD79a antibody [ZL7-4] (ab270256)

      Flow cytometric analysis of PFA-fixed Raji cells labeling CD79a using ab270256 at 2 μg/106 cells (Blue), followed by goat anti-mouse IgG-CF488; isotype control (Red).

    • Immunofluorescence - Anti-CD79a antibody [ZL7-4] (ab270256)
      Immunofluorescence - Anti-CD79a antibody [ZL7-4] (ab270256)

      Immunofluorescence analysis of PFA-fixed Raji cells labeling CD79a  using ab270256 at 2 μg/ml (Green), followed by goat anti-mouse IgG-CF488. The nuclear counterstain is Reddot (Red).

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270256 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270256 は論文での使用が確認できていません。

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