Anti-CD75 抗体 [B-L5] - BSA and Azide free (ab269707)
![Flow Cytometry - Anti-CD75 antibody [B-L5] - BSA and Azide free (ab269707)](https://www.abcam.co.jp/ps/products/269/ab269707/Images/ab269707-2-flowcytometrybloodcells.jpg "Flow Cytometry - Anti-CD75 antibody [B-L5] - BSA and Azide free (ab269707)")
Key features and details
- Mouse monoclonal [B-L5] to CD75 - BSA and Azide free
- Suitable for: Flow Cyt, IP
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-CD75 antibody [B-L5] - BSA and Azide free
CD75 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B-L5] to CD75 - BSA and Azide free -
由来種
Mouse -
特異性
This antibody recognises CD75.
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アプリケーション
適用あり: Flow Cyt, IPmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD75.
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特記事項
ab269707 is a carrier free version of ab27316. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
特記事項（精製）
This antibody was purified by gel filtration. -
ポリ/モノ
モノクローナル -
クローン名
B-L5 -
ミエローマ
P3x63-Ag8.653 -
アイソタイプ
IgM -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab269707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 10µl for 10 cells. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
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| IP | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 29 family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. -
細胞内局在
Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. - Information by UniProt
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参照データベース
- Entrez Gene: 6480 Human
- Omim: 109675 Human
- SwissProt: P15907 Human
- Unigene: 207459 Human
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別名
- 6 sialyltransferase antibody
- 6-sialyltransferase 1 antibody
- 6-ST 1 antibody
see all
画像
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Flow Cytometry - Anti-CD75 antibody [B-L5] - BSA and Azide free (ab269707)
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Immunoprecipitation - Anti-CD75 antibody (ab269707)CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 47kDa; CD75
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab27316).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab269707 は論文での使用が確認できていません。
