Anti-CD7 抗体 [Huly-m2] (Phycoerythrin) (ab1160)
製品の概要
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製品名Anti-CD7 antibody [Huly-m2] (Phycoerythrin)
CD7 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [Huly-m2] to CD7 (Phycoerythrin)
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由来種Mouse
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標識Phycoerythrin. Ex: 488nm, Em: 575nm
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特異性This antibody does not react with granulocytes and B lymphocytes.
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アプリケーション適用あり: Flow Cytmore details
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種交差性交差種: Human
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C.
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バッファーPreservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
ポリ/モノモノクローナル
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クローン名Huly-m2
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ミエローマunknown
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アイソタイプIgG2a
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軽鎖の種類unknown
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
追加情報
Diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL) andAcute myeloid leukaemia (CD7+ AML)in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
ターゲット情報
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機能Not yet known.
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配列類似性Contains 1 Ig-like (immunoglobulin-like) domain.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 924 Human
- Omim: 186820 Human
- SwissProt: P09564 Human
- Unigene: 36972 Human
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別名
- CD7 antibody
- CD7 antigen (p41) antibody
- CD7 antigen antibody
see all
プロトコール
参考文献
This product has been referenced in:
- Porwit-MacDonald A et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia 14:816-25 (2000). Read more (PubMed: 10803512) »
- Thurlow PJ et al. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38:143-7 (1984). Read more (PubMed: 6205484) »
