Anti-CD7 抗体 [Huly-m2] (Phycoerythrin) (ab1160)
製品の概要
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製品名
Anti-CD7 antibody [Huly-m2] (Phycoerythrin)
CD7 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Huly-m2] to CD7 (Phycoerythrin) -
由来種
Mouse -
標識
Phycoerythrin. Ex: 488nm, Em: 575nm -
特異性
This antibody does not react with granulocytes and B lymphocytes. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
Huly-m2 -
ミエローマ
unknown -
アイソタイプ
IgG2a -
軽鎖の種類
unknown -
研究分野
関連製品
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
追加情報
Diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL) andAcute myeloid leukaemia (CD7+ AML)in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
For FACS:
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
ターゲット情報
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機能
Not yet known. -
配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 924 Human
- Omim: 186820 Human
- SwissProt: P09564 Human
- Unigene: 36972 Human
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別名
- CD7 antibody
- CD7 antigen (p41) antibody
- CD7 antigen antibody
see all
プロトコール
参考文献
This product has been referenced in:
- Porwit-MacDonald A et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia 14:816-25 (2000). Read more (PubMed: 10803512) »
- Thurlow PJ et al. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38:143-7 (1984). Read more (PubMed: 6205484) »
