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リコンビナントRabMAb

Anti-CD7 抗体 [EPR4242] (ab109296)

Submit a review Submit a question References (1)
Western blot - Anti-CD7 antibody [EPR4242] (ab109296)
  • Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] (ab109296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
  • Immunocytochemistry - Anti-CD7 antibody [EPR4242] (ab109296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
  • Immunoprecipitation - Anti-CD7 antibody [EPR4242] (ab109296)
  • OI-RD Scanning - Anti-CD7 antibody [EPR4242] (ab109296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
  • Anti-CD7 antibody [EPR4242] (ab109296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4242] to CD7
  • Suitable for: ICC, WB, Flow Cyt (Intra), IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-CD7 antibody [EPR4242]
    CD7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4242] to CD7

  • 由来種

    Rabbit

  • 特異性

    The mouse and rat recommendation is based on the IHC-P results. We do not guarantee WB for mouse and rat.

  • アプリケーション

    適用あり: ICC, WB, Flow Cyt (Intra), IP, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt (intra): Jurkat cells; IP: Human spleen lysate; ICC: Jurkat cells; IHC-P: Rat and mouse spleen tissues. Human colon carcinoma and tonsil tissues; WB: Human spleen lysate,

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 解離定数（KD 値）

    KD = 2.40 x 10 -12 M
    Learn more about KD

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR4242

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109296の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
1/50.

For unpurified use at 1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
Flow Cyt (Intra)
1/100.
IP
1/10 - 1/100.
IHC-P
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/1600.
特記事項
ICC
1/50.

For unpurified use at 1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
Flow Cyt (Intra)
1/100.
IP
1/10 - 1/100.
IHC-P
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/1600.

ターゲット情報

画像

  • Western blot - Anti-CD7 antibody [EPR4242] (ab109296)
    Western blot - Anti-CD7 antibody [EPR4242] (ab109296)
    Anti-CD7 antibody [EPR4242] (ab109296) at 1/10000 dilution + Human Spleen Lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?



    The molecular mass observed is consistent with what has been described in the literatures (PMID: 24157461, 2479685).

  • Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] (ab109296)
    Flow Cytometry (Intracellular) - Anti-CD7 antibody [EPR4242] (ab109296)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD7 with purified ab109296 at 1/100 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunocytochemistry - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD7 with Purified ab109296 at 1/50 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD7 with Purified ab109296 at 1/8000 dilution (0.07 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunoprecipitation - Anti-CD7 antibody [EPR4242] (ab109296)

    Purified ab131366 at 1/50 dilution immunoprecipitating CD7 in Human spleen whole cell lysate.

    Lane 1 (input): Human spleen lysate 10 μg
    Lane 2 (+): Human spleen lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109296 in Human spleen lysate

    For western blotting, ab227648 at 1/1000 dilution (0.45 μg/ml) and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/50000 dilution were used.
    Blocking and diluting buffer: 5% NFDM /TBST.

  • OI-RD Scanning - Anti-CD7 antibody [EPR4242] (ab109296)
    OI-RD Scanning - Anti-CD7 antibody [EPR4242] (ab109296)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD7 antibody [EPR4242] (ab109296)

    Tissue Microarrays stained for "Anti-CD7 antibody [EPR4242]” using "ab109296" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab109296 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-CD7 antibody [EPR4242] (ab109296)
    Anti-CD7 antibody [EPR4242] (ab109296)

参考文献 (1)

ab109296 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109296 は 1 報の論文で使用されています。

  • Ng SY  et al. Targetable vulnerabilities in T- and NK-cell lymphomas identified through preclinical models. Nat Commun 9:2024 (2018). PubMed: 29789628

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