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Anti-CD6/T12 抗体 [C6/2884R] - BSA and Azide free (ab270302)

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Immunocytochemistry/ Immunofluorescence - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)
  • Flow Cytometry - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)
  • Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [C6/2884R] to CD6/T12 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free
    CD6/T12 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [C6/2884R] to CD6/T12 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Recombinant full length protein within Human CD6/T12. The exact sequence is proprietary.
    Database link: P30203

  • ポジティブ･コントロール

    • IHC-P: Human tonsil tissue. Flow Cyt: MOLT-4 cells. ICC/IF: MOLT-4 cells.

  • 特記事項

    ab270302 is a carrier free version of ab270271.

     

製品の特性

関連製品

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
ICC/IF
Use a concentration of 1 - 2 µg/ml.
Flow Cyt
Use 1-2µg for 106 cells.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Involved in cell adhesion. Binds to CD166.

  • 組織特異性

    Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.

  • 配列類似性

    Contains 3 SRCR domains.

  • 翻訳後修飾

    After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
    Contains intrachain disulfide bond(s).

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD_antigen=CD6 antibody
      • CD6 antibody
      • CD6 antigen Tp120 antibody
      • CD6 molecule antibody
      • CD6_HUMAN antibody
      • FLJ44171 antibody
      • OX52 antibody
      • T cell differentiation antigen CD6 antibody
      • T-cell differentiation antigen CD6 antibody
      • T12 antibody
      • TP120 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)
      Immunocytochemistry/ Immunofluorescence - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)

      Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia cell line) cells labeling CD6/T12 (green) using the ab270271 at 2µg/ml, followed by goat anti-rabbit IgG to conjugate with CF488 (green). The nuclear counterstain is Reddot (red).

      This image was produced using the same antibody clone but in a different formulation ab270271, PBS, BSA and sodium azide.

    • Flow Cytometry - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)
      Flow Cytometry - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)

      Flow cytometric analsysis of MOLT-4 (Human lymphoblastic leukemia cell line) cells labeling CD6/T12 using ab270271 at 2µg/10^6 cells followed by the goat anti-rabbit IG-CF488 (blue); isotope control (red).

      This image was produced using the same antibody clone but in a different formulation ab270271, PBS, BSA and sodium azide.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD6/T12 using ab270271 at 2µg/ml in immunohistochemical analyisis.

      This image was produced using the same antibody clone but in a different formulation ab270271, PBS, BSA and sodium azide.

    • Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)
      Anti-CD6/T12 antibody [C6/2884R] - BSA and Azide free (ab270302)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270302 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270302 は論文での使用が確認できていません。

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