製品の概要
-
製品名Anti-CD68 antibody [PG-M1], prediluted
CD68 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [PG-M1] to CD68, prediluted
-
由来種Mouse
-
特異性This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.
-
アプリケーション適用あり: IHC-Pmore details
-
種交差性交差種: Human
-
免疫原
BALB/C mice were immunized with Gaucher’s cells.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
-
バッファーPreservative: 0.05% Sodium Azide
Constituents: Inert stabilizer -
Concentration information loading... -
ポリ/モノモノクローナル
-
クローン名PG-M1
-
ミエローマunknown
-
アイソタイプIgG3
-
軽鎖の種類kappa
-
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
追加情報
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
This antibody is not recommended for frozen sections or cell smears.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
This antibody is not recommended for frozen sections or cell smears.
ターゲット情報
-
機能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
-
組織特異性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
-
配列類似性Belongs to the LAMP family.
-
翻訳後修飾N- and O-glycosylated.
-
細胞内局在Cell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
-
参照データベース
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
-
別名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
画像
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)](https://www.abcam.co.jp/ps/datasheet/images/0/ab844/CD68-Primary-antibodies-ab844-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)ab844 staining CD68 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.
参考文献
This product has been referenced in:
- Tewari A et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF . Read more (PubMed: 24714202) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"