Anti-CD68 抗体 [PG-M1], prediluted (ab844)

Reviews (1)Q&A (1)References (1)

製品の概要

  • 製品名

    Anti-CD68 antibody [PG-M1], prediluted
    CD68 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [PG-M1] to CD68, prediluted

  • 由来種

    Mouse

  • 特異性

    This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    BALB/C mice were immunized with Gaucher’s cells.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    Preservative: 0.05% Sodium azide

    Inert stabilizer
  • Concentration information loading...

  • ポリ/モノ

    モノクローナル

  • クローン名

    PG-M1

  • ミエローマ

    unknown

  • アイソタイプ

    IgG3

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
    This antibody is not recommended for frozen sections or cell smears.

    • ターゲット情報

    • 機能

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

    • 組織特異性

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

    • 配列類似性

      Belongs to the LAMP family.

    • 翻訳後修飾

      N- and O-glycosylated.

    • 細胞内局在

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)
        ab844 staining CD68 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.

      プロトコール

      参考文献

      This product has been referenced in:

      • Tewari A  et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF . Read more (PubMed: 24714202) »
      See 1 Publication for this product

      ab844 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1], prediluted

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Spleen)
      Specification
      Spleen
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
      Read More

      Mr. Carl Hobbs

      Verified customer

      投稿 Sep 06 2012

      Question

      Telefon call:

      Wants to label ab783 or ab844 with AF488,
      What is in the buffer of ab844? Will it interfere with the labeling reaction, what is the concentration?

      What is the concentration of ab783?

      Read More
      Answer

      Thank you for your inquiry.

      I apologise for the delay. I had to wait for an answer from the source of these two products.

      I can now confirm that the concentration of ab783 is 10ug/ml and ab844 is approximately 2-4ug/ml.

      They also contain 0.05% sodium azide which might interfere with the conjugation reaction.

      I hope this information is helpful and wish you a good weekend.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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