アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    cd68-antibody-pg-m1-ab783.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Cell Type Markers CD Myeloid Cells
Share by email

Anti-CD68 抗体 [PG-M1] (ab783)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (8)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)

Key features and details

  • Mouse monoclonal [PG-M1] to CD68
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG3

こちらの製品もご検討ください

一次抗体
Product image
Anti-CD16 antibody [SP175] (ab183354)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一次抗体
Product image
Anti-FOXP3 antibody [236A/E7] - BSA and Azide free (ab96048)

関連製品

製品の概要

  • 製品名

    Anti-CD68 antibody [PG-M1]
    CD68 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [PG-M1] to CD68
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ICC or IHC-Fr
  • 種交差性

    交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to CD68. Gaucher cells

  • ポジティブ・コントロール

    • IHC-P: Human tonsil and granulomatous inflammatory tissues.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 98.95% Tissue culture supernatant, 1% BSA
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    PG-M1
  • アイソタイプ

    IgG3
  • 軽鎖の種類

    kappa
  • 研究分野

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Neuroscience
    • Development

関連製品

  • Alternative Versions

    • Anti-CD68 antibody [PG-M1], prediluted (ab844)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)
  • Recombinant Protein

    • Recombinant human CD68 protein (Fc Chimera Active) (ab214829)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab783の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (2)
1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature.
特記事項
IHC-P
1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature.
追加情報
Is unsuitable for ICC or IHC-Fr.

ターゲット情報

  • 機能

    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 組織特異性

    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 配列類似性

    Belongs to the LAMP family.
  • 翻訳後修飾

    N- and O-glycosylated.
  • 細胞内局在

    Cell membrane and Endosome membrane. Lysosome membrane.
  • Target information above from: UniProt accession P34810 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 968 Human
    • Omim: 153634 Human
    • SwissProt: P34810 Human
    • Unigene: 647419 Human
    • 別名

      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)

      ab783 staining CD68 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)This image is courtesy of an anonymous Abreview.
      Paraformaldehyde-fixed, paraffin-embedded human granulomatous inflammatory tissue stained for CD68 using ab783 in immunohistochemical analysis.

      See Abreview

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (8)

    ab783 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab783 は 8 報の論文で使用されています。

    • Yamada K  et al. Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer. Oncol Lett 21:10 (2021). PubMed: 33240416
    • Waller R  et al. Iba-1-/CD68+ microglia are a prominent feature of age-associated deep subcortical white matter lesions. PLoS One 14:e0210888 (2019). PubMed: 30682074
    • Pratt D  et al. Programmed Death Ligand 1 Is a Negative Prognostic Marker in Recurrent Isocitrate Dehydrogenase-Wildtype Glioblastoma. Neurosurgery N/A:N/A (2018). IHC-P . PubMed: 30011045
    • Liu JYW  et al. Doublecortin-expressing cell types in temporal lobe epilepsy. Acta Neuropathol Commun 6:60 (2018). PubMed: 30005693
    • Hultman K  et al. The APOE ?4/?4 genotype potentiates vascular fibrin(ogen) deposition in amyloid-laden vessels in the brains of Alzheimer's disease patients. J Cereb Blood Flow Metab 33:1251-8 (2013). PubMed: 23652625
    • Horny HP  et al. Immunoreactivity of normal and neoplastic human tissue mast cells with macrophage-associated antibodies, with special reference to the recently developed monoclonal antibody PG-M1. Hum Pathol 24:355-8 (1993). PubMed: 8491475
    • Kaiserling E  et al. Aberrant expression of macrophage-associated antigens (CD68 and Ki-M1P) by Schwann cells in reactive and neoplastic neural tissue. Light- and electron-microscopic findings. Mod Pathol 6:463-8 (1993). PubMed: 8415593
    • Thiele J  et al. Macrophages in normal human bone marrow and in chronic myeloproliferative disorders: an immunohistochemical and morphometric study by a new monoclonal antibody (PG-M1) on trephine biopsies. Virchows Arch A Pathol Anat Histopathol 421:33-9 (1992). PubMed: 1636247

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Meninges)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
    Permeabilization
    Yes - 0.1% tween in PBS
    Specification
    Meninges
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 02 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (granulomatous inflammatory tissue)
    Specification
    granulomatous inflammatory tissue
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Pepsin
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 21 2008

    Question

    Telefon call:

    Wants to label ab783 or ab844 with AF488,
    What is in the buffer of ab844? Will it interfere with the labeling reaction, what is the concentration?

    What is the concentration of ab783?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 15 2012

    Answer

    Thank you for your inquiry.

    I apologise for the delay. I had to wait for an answer from the source of these two products.

    I can now confirm that the concentration of ab783 is 10ug/ml and ab844 is approximately 2-4ug/ml.

    They also contain 0.05% sodium azide which might interfere with the conjugation reaction.

    I hope this information is helpful and wish you a good weekend.

    Read More

    Abcam Scientific Support

    Answered on Jun 15 2012

    Question

    We are looking for antibodies to easily tell the difference between astocytes, oligodendrocytes and macrophages (including microglia) in mature human brain tissues. Goal is to use them in double immunohistochemistry staining on formalin fixed, paraffin embedded tissues. Is this CD68 antibody proper for this purpose ?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 21 2005

    Answer

    Thank you for your enquiry regarding ab783. We have received feedback from the source of the antibody telling us that "this CD68 is specific against macrophages only" unfortunately. I would like to recommend looking at the following page from our website to help you find markers of each cell population: http://abcam2/index.html?pageconfig=resource&rid=295&pid=7 I hope this information will help you, please do not hesitate to contact us again if you need further assistance,

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.