Anti-CD68 抗体 [PG-M1] (ab783)
Key features and details
- Mouse monoclonal [PG-M1] to CD68
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG3
製品の概要
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製品名
Anti-CD68 antibody [PG-M1]
CD68 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PG-M1] to CD68 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details
適用なし: ICC or IHC-Fr -
種交差性
交差種: Human -
免疫原
Gaucher cells
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ポジティブ･コントロール
- IHC-P: Human tonsil and granulomatous inflammatory tissues.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
PG-M1 -
アイソタイプ
IgG3 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature. |
追加情報
Is unsuitable for ICC or IHC-Fr.
ターゲット情報
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機能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
組織特異性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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別名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)](https://www.abcam.co.jp/ps/products/0/ab783/Images/ab783-289954-anti-cd68-antibody-pg-m1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)ab783 staining CD68 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)](https://www.abcam.co.jp/ps/products/0/ab783/Images/ab783-291423-anti-cd68-antibody-pg-m1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)This image is courtesy of an anonymous Abreview.Paraformaldehyde-fixed, paraffin-embedded human granulomatous inflammatory tissue stained for CD68 using ab783 in immunohistochemical analysis.
参考文献
ab783 は 7 報の論文で使用されています。
- Waller R et al. Iba-1-/CD68+ microglia are a prominent feature of age-associated deep subcortical white matter lesions. PLoS One 14:e0210888 (2019). PubMed: 30682074
- Pratt D et al. Programmed Death Ligand 1 Is a Negative Prognostic Marker in Recurrent Isocitrate Dehydrogenase-Wildtype Glioblastoma. Neurosurgery N/A:N/A (2018). IHC-P . PubMed: 30011045
- Liu JYW et al. Doublecortin-expressing cell types in temporal lobe epilepsy. Acta Neuropathol Commun 6:60 (2018). PubMed: 30005693
- Hultman K et al. The APOE ?4/?4 genotype potentiates vascular fibrin(ogen) deposition in amyloid-laden vessels in the brains of Alzheimer's disease patients. J Cereb Blood Flow Metab 33:1251-8 (2013). PubMed: 23652625
- Horny HP et al. Immunoreactivity of normal and neoplastic human tissue mast cells with macrophage-associated antibodies, with special reference to the recently developed monoclonal antibody PG-M1. Hum Pathol 24:355-8 (1993). PubMed: 8491475
- Kaiserling E et al. Aberrant expression of macrophage-associated antigens (CD68 and Ki-M1P) by Schwann cells in reactive and neoplastic neural tissue. Light- and electron-microscopic findings. Mod Pathol 6:463-8 (1993). PubMed: 8415593
- Thiele J et al. Macrophages in normal human bone marrow and in chronic myeloproliferative disorders: an immunohistochemical and morphometric study by a new monoclonal antibody (PG-M1) on trephine biopsies. Virchows Arch A Pathol Anat Histopathol 421:33-9 (1992). PubMed: 1636247
