Anti-CD68 抗体 [PG-M1] (ab783)

Reviews (2)Q&A (2)References (7)

製品の概要

  • 製品名

    Anti-CD68 antibody [PG-M1]
    CD68 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [PG-M1] to CD68

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ICC or IHC-Fr

  • 種交差性

    交差種: Human

  • 免疫原

    Gaucher cells

  • ポジティブ･コントロール

    • IHC-P: Human tonsil and granulomatous inflammatory tissues.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    PG-M1

  • アイソタイプ

    IgG3

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature.
  • 追加情報
    Is unsuitable for ICC or IHC-Fr.

    • ターゲット情報

    • 機能

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

    • 組織特異性

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

    • 配列類似性

      Belongs to the LAMP family.

    • 翻訳後修飾

      N- and O-glycosylated.

    • 細胞内局在

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)

        ab783 staining CD68 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)This image is courtesy of an anonymous Abreview.
        Paraformaldehyde-fixed, paraffin-embedded human granulomatous inflammatory tissue stained for CD68 using ab783 in immunohistochemical analysis.

        See Abreview

      プロトコール

      参考文献

      This product has been referenced in:

      • Waller R  et al. Iba-1-/CD68+ microglia are a prominent feature of age-associated deep subcortical white matter lesions. PLoS One 14:e0210888 (2019). Read more (PubMed: 30682074) »
      • Pratt D  et al. Programmed Death Ligand 1 Is a Negative Prognostic Marker in Recurrent Isocitrate Dehydrogenase-Wildtype Glioblastoma. Neurosurgery N/A:N/A (2018). IHC-P . Read more (PubMed: 30011045) »
      See all 7 Publications for this product

      ab783 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

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      1-4 of 4 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Meninges)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
      Permeabilization
      Yes - 0.1% tween in PBS
      Specification
      Meninges
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Jan 02 2020

      Question

      Telefon call:

      Wants to label ab783 or ab844 with AF488,
      What is in the buffer of ab844? Will it interfere with the labeling reaction, what is the concentration?

      What is the concentration of ab783?

      Read More
      Answer

      Thank you for your inquiry.

      I apologise for the delay. I had to wait for an answer from the source of these two products.

      I can now confirm that the concentration of ab783 is 10ug/ml and ab844 is approximately 2-4ug/ml.

      They also contain 0.05% sodium azide which might interfere with the conjugation reaction.

      I hope this information is helpful and wish you a good weekend.

      Read More

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (granulomatous inflammatory tissue)
      Specification
      granulomatous inflammatory tissue
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Enzymatic - Buffer/Enzyme Used: Pepsin
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      投稿 Jul 21 2008

      Question

      We are looking for antibodies to easily tell the difference between astocytes, oligodendrocytes and macrophages (including microglia) in mature human brain tissues. Goal is to use them in double immunohistochemistry staining on formalin fixed, paraffin embedded tissues. Is this CD68 antibody proper for this purpose ?

      Read More
      Answer

      Thank you for your enquiry regarding ab783. We have received feedback from the source of the antibody telling us that "this CD68 is specific against macrophages only" unfortunately. I would like to recommend looking at the following page from our website to help you find markers of each cell population: http://abcam2/index.html?pageconfig=resource&rid=295&pid=7 I hope this information will help you, please do not hesitate to contact us again if you need further assistance,

      Read More

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