Key features and details
- Mouse monoclonal [KP1] to CD68, prediluted
- Suitable for: Flow Cyt, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品名Anti-CD68 antibody [KP1], prediluted
CD68 一次抗体 製品一覧
製品の詳細Mouse monoclonal [KP1] to CD68, prediluted
特異性This antibody is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
アプリケーション適用あり: Flow Cyt, IHC-P, IHC-Fr, ICC/IFmore details
Subcellular fraction of human alveolar macrophages.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
バッファーPreservative: 0.05% Sodium azide
Concentration information loading...
特記事項（精製）Purified from TCS
Our Abpromise guarantee covers the use of ab845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. High temperature antigen unmasking may improve the staining.
|IHC-Fr||Use at an assay dependent concentration. Acetone fixed.|
|ICC/IF||Use at an assay dependent concentration.|
機能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
組織特異性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
配列類似性Belongs to the LAMP family.
翻訳後修飾N- and O-glycosylated.
細胞内局在Cell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
Overlay histogram showing THP1 cells stained with ab845 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab845, 1/4 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab845 は 11 報の論文で使用されています。
- Wu SQ et al. Prognostic roles of tumor associated macrophages in bladder cancer: a system review and meta-analysis. Oncotarget 9:25294-25303 (2018). PubMed: 29861872
- Zeis T et al. Molecular pathology of Multiple Sclerosis lesions reveals a heterogeneous expression pattern of genes involved in oligodendrogliogenesis. Exp Neurol 305:76-88 (2018). IHC-Fr ; Human . PubMed: 29596844
- Ramakrishnan KA et al. Anticytokine autoantibodies in a patient with a heterozygous NFKB2 mutation. J Allergy Clin Immunol 141:1479-1482.e6 (2018). PubMed: 29225085
- Ren CX et al. Intratumoral and peritumoral expression of CD68 and CD206 in hepatocellular carcinoma and their prognostic value. Oncol Rep 38:886-898 (2017). PubMed: 28656201
- Tervahartiala M et al. Immunological tumor status may predict response to neoadjuvant chemotherapy and outcome after radical cystectomy in bladder cancer. Sci Rep 7:12682 (2017). IHC-P ; Human . PubMed: 28978923