Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 10.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Horse serum
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Vector Immpress
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Developed with DAB (instant) and counterstained with Harris Hematoxylin. Tried antigen retrieval with both Sodium Citrate and Citric Acid buffers (pH 6.0), but those retrieved in Tris (pH 10.0) showed the clearest staining. 40X image.
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投稿 May 03 2013