製品の概要

  • 製品名
    Anti-CD68 antibody [KP1]
    CD68 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [KP1] to CD68
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit
  • ポジティブ・コントロール
    • Tonsil

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.099% Sodium azide
    Constituents: 0.9% Proprietary component, 99% Water
  • Concentration information loading...
  • 精製度
    Protein A/G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    KP1
  • ミエローマ
    unknown
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 18804859
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Recommended heat mediated antigen retrieval in pressure cooker for 2-5 minutes or steaming of tissue sections for 45-60 minutes followed by cooling for 10 minutes and washing in distilled water.

In case of enzymatic epitope retrieval - digest with Pepsin enzyme for 30-60 seconds at RT.

IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 組織特異性
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 配列類似性
    Belongs to the LAMP family.
  • 翻訳後修飾
    N- and O-glycosylated.
  • 細胞内局在
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

画像

  • ab955 staining CD68 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in antibody diution reagent) for 30 minutes at 20°C. An undiluted HRP-conjugated Rat anti-mouse/rabbit polymer was used as the secondary antibody.

    See Abreview

  • ab955 staining CD68 in Human ulcerated Oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

    See Abreview

  • ab955 staining CD68 in Human lung cancer tissues by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 2% horse serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI containing mounting medium was used for nuclear staining (blue) and anti-TREM-1 (1/500) used for TREM-1 staining (red)

  • Immunohistochemical analysis of tonsil labelling CD68 with ab955.

  • ab955 at a 1/2000 dilution staining CD68 from mouse lung tissue sections (new born pups) by immunohistochemistry (frozen sections). The antibody was incubated with the tissue for 24 hours and then detected using an Alexa-Fluor® 488 Goat anti-mouse IgG. The negative control was secondary antibody alone.

    See Abreview

  • ab955 staining CD68 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% horse serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Tris buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 15 hours at 4°C. An undiluted HRP-conjugated Horse polyclonal was used as the secondary antibody.

    See Abreview

  • ab955 staining CD68 in Rabbit iliac tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in Dako antibody diluent) for 4 hours at 25°C. An undiluted Beta-galactosidase-conjugated rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • Formalin-fixed, paraffin embedded sections of mouse spleen were stained for CD68 with ab at 1/200 dilution in immunohistochemical analysis. Alexa Fluor 647® conjugated Donkey Anti-Mouse was used as secondary antibody at 1/200 dilution.

  • Immunohistochemical analysis of tonsil labelling CD68 with ab955.

  • ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • You S  et al. An Aza resveratrol-chalcone derivative 6b protects mice against diabetic cardiomyopathy by alleviating inflammation and oxidative stress. J Cell Mol Med 22:1931-1943 (2018). Read more (PubMed: 29327811) »
  • Kim SJ  et al. Anti-inflammatory effect of Tauroursodeoxycholic acid in RAW 264.7 macrophages, Bone marrow-derived macrophages, BV2 microglial cells, and spinal cord injury. Sci Rep 8:3176 (2018). Read more (PubMed: 29453346) »

See all 129 Publications for this product

レビューと Q&A

The IgG concentration of ab955, Anti-CD68 antibody [KP1], lot GR173584-2 is 4µg/mL.



Any products that require an MSDS form will have a link on the product's online datasheet to the data. It will be a PDF document link labeled 'SDS'. Please see attached image for example of what to look for on the datasheet. If no 'SDS' link ...

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Thank you very much for your calls this week and for letting us know about the trouble with this antibody in IHC-Fr.

As we discussed, I'm sending a free of charge replacement vial of ab125212 on the order ***. This should arrive on Monday. Read More

Thank you for bringing this to our attention. Please contact me if the cold acetone (on ice, 10 minutes) fixation that I recommended does not improve your result.

Thank you for your enquiry.

I can confirm that the immunogen used for CD68 antibody [KP1] (ab955) is lysosomal fraction of human lung macrophages.

I am sorry this has not been specifically epitope mapped and we have no further inf...

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Muchas gracias por tu respuesta.

Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Est...

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Thank you for contacting us.

Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

However, instead of using PFA as a fixative, I w...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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Thank you very much for your interest in anti-CD68 antibody clone [KP1] (ab955).

To our knowledge, ab955 has not been tested in Western blot, but I do not see a reason why it should not work. Therefore, I can offer a discount off a future pu...

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Thank you for contacting Abcam.

I feel that both products are suitable for IHC-P. Both have many specific references and in-depth scientific support data right on the datasheet. They differ mainly in the species in which they were raised and i...

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1-10 of 16 Q&A

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