Anti-CD68 抗体 [KP1] (ab955)

ab955 staining CD68 in rabbit iliac tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 4 hours at 25°C. An undiluted beta-galactosidase-conjugated rabbit polyclonal was used as the secondary antibody.

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ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor^® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

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ab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

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ab955 staining CD68 in human lung cancer tissues by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with formalin and blocked with 2% horse serum for 1 hour at room temperature. Samples were then incubated with primary antibody (1/100) for 1 hour at 21°C. An Alexa Fluor^® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI containing mounting medium was used for nuclear staining (blue) and anti-TREM-1 (1/500) used for TREM-1 staining (red).

ab955 staining CD68 in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti-mouse/rabbit polymer was used as the secondary antibody.

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Immunohistochemical analysis of tonsil labeling CD68 with ab955.

ab955 at a 1/2000 dilution staining CD68 from mouse lung tissue sections (new born pups) by immunohistochemistry (frozen sections).

The antibody was incubated with the tissue for 24 hours and then detected using an Alexa-Fluor^® 488 Goat anti-mouse IgG.

The negative control was secondary antibody alone.

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ab955 staining CD68 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with paraformaldehyde and blocked with 5% horse serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Tris buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 15 hours at 4°C. An undiluted HRP-conjugated horse polyclonal was used as the secondary antibody.

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Formalin-fixed, paraffin embedded sections of mouse spleen were stained for CD68 with ab at 1/200 dilution in immunohistochemical analysis. Alexa Fluor 647^® conjugated Donkey Anti-Mouse was used as secondary antibody at 1/200 dilution.

Immunohistochemical analysis of tonsil labeling CD68 with ab955.