Anti-CD68 抗体 [KP1] (ab955)
![Western blot - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.co.jp/ps/products/0/ab955/Images/ab955-414959-AntiCD68antibodyWesternblot.jpg "Western blot - Anti-CD68 antibody [KP1] (ab955)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [KP1] to CD68
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD68 antibody [KP1]
CD68 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [KP1] to CD68 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit
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ポジティブ･コントロール
- IHC-P: human tonsil and liver tissues; ICC: THP-1 cells; WB: Human spleen lysate.
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特記事項
Anti CD68 antibody (ab955) is reccomended for IHC on human samples but is not reccomended for mouse & rat samples.
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
KP1 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Antibody Diluent
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab955の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 37 kDa.
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| ICC |
1/50.
|
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| IHC-P | (29) |
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 37 kDa. |
|
ICC
1/50. |
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IHC-P
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
組織特異性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 968 Human
- Entrez Gene: 12514 Mouse
- Entrez Gene: 287435 Rat
- Omim: 153634 Human
- SwissProt: P34810 Human
- SwissProt: P31996 Mouse
- Unigene: 647419 Human
- Unigene: 15819 Mouse
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別名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
画像
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Western blot - Anti-CD68 antibody [KP1] (ab955)Anti-CD68 antibody [KP1] (ab955) at 1/1000 dilution + Human spleen lysate at 20 µg
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG(H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsThe observed molecular weight is consistent with the literature (PMID:18405323; PMID:11739566; PMID: 16710801).
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![Immunocytochemistry - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.co.jp/ps/products/0/ab955/Images/ab955-414752-AntiCD68antibodyImmunocytochemistry.jpg)
Immunocytochemistry - Anti-CD68 antibody [KP1] (ab955)Immunocytochemistry analysis of THP-1 (human monocytic leukemia monocyte) labelling CD28 with ab955 at 1/50 dilution. Cells were fixed with 100% methanol. Goat Anti-mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Tubulin antibody (rabbit mAb), ab179504 - AlexaFluor®594 Goat anti- Rabbit secondary, ab150080 at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing cytoplasmic staining in THP-1 cells
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.co.jp/ps/products/0/ab955/Images/ab955-414750-AntiCD68antibodyImmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.co.jp/ps/products/0/ab955/Images/ab955-414749-AntiCD68antibodyImmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.co.jp/ps/products/0/ab955/Images/ab955-3836-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Image courtesy of an anonymous Abreview.ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.co.jp/ps/products/0/ab955/Images/ab955-3837-anti-cd68-antibody-kp1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)This image is courtesy of an anonymous Abreviewab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.
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![Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.co.jp/ps/products/0/ab955/Images/ab955-18-benefits-of-recombinant-antibodies.png)
Anti-CD68 antibody [KP1] (ab955)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (354)
ab955 は 354 報の論文で使用されています。
- Hou C et al. Mono-macrophage-Derived MANF Protects Against Lipopolysaccharide-Induced Acute Kidney Injury via Inhibiting Inflammation and Renal M1 Macrophages. Inflammation 44:693-703 (2021). PubMed: 33145627
- Finlin BS et al. The ß3-adrenergic receptor agonist mirabegron improves glucose homeostasis in obese humans. J Clin Invest 130:2319-2331 (2020). PubMed: 31961829
- Atsumi S et al. Focal adhesion ribonucleoprotein complex proteins are major humoral cancer antigens and targets in autoimmune diseases. Commun Biol 3:588 (2020). PubMed: 33067514
- Kvorjak M et al. Cross-talk between Colon Cells and Macrophages Increases ST6GALNAC1 and MUC1-sTn Expression in Ulcerative Colitis and Colitis-Associated Colon Cancer. Cancer Immunol Res 8:167-178 (2020). PubMed: 31831633
- Liang H et al. Topical nanoparticles interfering with the DNA-LL37 complex to alleviate psoriatic inflammation in mice and monkeys. Sci Adv 6:eabb5274 (2020). PubMed: 32923608