Alexa Fluor® 488 Anti-CD68 抗体 [FA-11] (ab201844)
Key features and details
- Alexa Fluor® 488 Rat monoclonal [FA-11] to CD68
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 488 Anti-CD68 antibody [FA-11]
CD68 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Rat monoclonal [FA-11] to CD68 -
由来種
Rat -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: RAW246.7 cells
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特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
FA-11 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 405 Anti-CD68 antibody [FA-11] (ab199571)
- Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
- HRP Anti-CD68 antibody [FA-11] (ab216526)
- PE Anti-CD68 antibody [FA-11] (ab216701)
- PerCP Anti-CD68 antibody [FA-11] (ab220509)
- APC Anti-CD68 antibody [FA-11] (ab221251)
- Anti-CD68 antibody [FA-11] - BSA and Azide free (ab237968)
- Anti-CD68 antibody [FA-11] (ab53444)
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab201844の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/250.
This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min) |
特記事項 |
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ICC/IF
1/250. This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min) |
ターゲット情報
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機能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
組織特異性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12514 Mouse
- SwissProt: P31996 Mouse
- Unigene: 15819 Mouse
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別名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
画像
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ab201844 staining CD68 in RAW264.7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201844 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab201844 は 9 報の論文で使用されています。
- Zolfaghari S et al. Tissue distribution and transcriptional regulation of CCN5 in the heart after myocardial infarction. J Cell Commun Signal 16:377-395 (2022). PubMed: 34854055
- Götz P et al. Cobra Venom Factor Boosts Arteriogenesis in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35955584
- Ma L et al. Tanyu Tongzhi Formula Delays Atherosclerotic Plaque Progression by Promoting Alternative Macrophage Activation via PPARγ and AKT/ERK Signal Pathway in ApoE Knock-Out Mice. Front Pharmacol 12:734589 (2021). PubMed: 34966274
- Bobba CM et al. Nanoparticle delivery of microRNA-146a regulates mechanotransduction in lung macrophages and mitigates injury during mechanical ventilation. Nat Commun 12:289 (2021). PubMed: 33436554
- Götz P et al. C3 Deficiency Leads to Increased Angiogenesis and Elevated Pro-Angiogenic Leukocyte Recruitment in Ischemic Muscle Tissue. Int J Mol Sci 22:N/A (2021). PubMed: 34071589
- Kimm MA et al. Targeting Endothelin Receptors in a Murine Model of Myocardial Infarction Using a Small Molecular Fluorescent Probe. Mol Pharm 17:109-117 (2020). PubMed: 31816245
- Kumaraswami K et al. A Simple and Effective Flow Cytometry-Based Method for Identification and Quantification of Tissue Infiltrated Leukocyte Subpopulations in a Mouse Model of Peripheral Arterial Disease. Int J Mol Sci 21:N/A (2020). PubMed: 32438752
- Lasch M et al. RNase A Treatment Interferes With Leukocyte Recruitment, Neutrophil Extracellular Trap Formation, and Angiogenesis in Ischemic Muscle Tissue. Front Physiol 11:576736 (2020). PubMed: 33240100
- Yang J et al. The influence of PM2.5 on lung injury and cytokines in mice. Exp Ther Med 18:2503-2511 (2019). PubMed: 31572502