Anti-CD68 抗体 [C68/684] (ab201340)
Key features and details
- Mouse monoclonal [C68/684] to CD68
- Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD68 antibody [C68/684]
CD68 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [C68/684] to CD68 -
由来種
Mouse -
特異性
ab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68. -
アプリケーション
適用あり: WB, Flow Cyt, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Monkey -
免疫原
Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).
Database link: P34810 -
ポジティブ・コントロール
- IHC-P: Human tonsil and histiocytoma tissues; ICC/IF: U-87 MG cells; Flow Cyt: U-87 MG cells; WB: Human spleen tissue lysate.
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特記事項
ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
ab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
ポリ/モノ
モノクローナル -
クローン名
C68/684 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab201340の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
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Flow Cyt | (1) |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P | (2) |
Use a concentration of 1 - 2 µg/ml.
Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Incubate with primary for 30 minutes at RT. |
ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa). |
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Incubate with primary for 30 minutes at RT. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
組織特異性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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別名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.
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Flow Cytometry analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (blue); Isotype Control (red).
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Immunocytochemistry/immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labelling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (green). Nuclear DNA stained red.
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Anti-CD68 antibody [C68/684] (ab201340) + Human spleen tissue lysate
Predicted band size: 37 kDa
Additional bands at: 110 kDa (possible glycosylated form)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (81)
ab201340 は 81 報の論文で使用されています。
- Tsai MT et al. Plasma Galectin-9 Is a Useful Biomarker for Predicting Renal Function in Patients Undergoing Native Kidney Biopsy. Arch Pathol Lab Med 147:167-176 (2023). PubMed: 35687787
- Wang Y et al. Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections. J Control Release 349:118-132 (2022). PubMed: 35792186
- Shi C et al. SIRT3 inhibitor 3-TYP exacerbates thioacetamide-induced hepatic injury in mice. Front Physiol 13:915193 (2022). PubMed: 35923224
- Deng Y et al. Hyperhomocysteinemia Promotes Cardiac Hypertrophy in Hypertension. Oxid Med Cell Longev 2022:1486157 (2022). PubMed: 36046692
- Yuan X et al. RGMa promotes dedifferentiation of vascular smooth muscle cells into a macrophage-like phenotype in vivo and in vitro. J Lipid Res 63:100276 (2022). PubMed: 36089003